DGK-theta Antibody (652308) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 652308
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

DGK-theta Antibody (652308) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human DGK-θ
  Asp696-Lys839
  Accession # P52824
• Specificity: Detects DGK-theta in direct ELISAs and Western blots. In direct ELISAs and Western blolts, no cross-reactivity with recombinant human DGK-alpha , -beta , -gamma , -epsilon , -iota , -kappa , or -zeta is observed.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-theta Antibody (652308) [Alexa Fluor® 488]

• DAG kinase theta
• DAGK
• DAGK4EC 2.7.1.107
• DAGK7
• DGKQ
• DGKtheta
• DGK-theta
• diacylglycerol kinase theta
• diacylglycerol kinase, theta (110kD)
• diacylglycerol kinase, theta 110kDa
• Diglyceride kinase theta

Background

Diacylglycerol kinase theta (DGK-theta ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-theta binds to RhoA, Akt, PKC epsilon and eta, and Steroidogenic Factor. It translocates to the nucleus or to the plasma membrane in response to NGF, TPA, or GPCR stimulation (thrombin, bradykinin, norepinephrine). DGK-theta contains three Cys-rich zinc finger regions (aa 60-108, aa 121-168, and aa 183-234), a Ras-associating domain (aa 395-494), two LxxLL motifs (aa 555-559 and aa 574-578), and a catalytic domain (aa 590-714). Over aa 696-839, human DGK-theta shares 90% aa sequence identity with mouse and rat DGK-theta .

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.