DGK-iota Antibody (648538)

Western blot shows lysates of SH‑SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK‑ iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK‑ iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 648538
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DGK-iota Antibody (648538) Summary

• Immunogen: E. coli-derived recombinant human DGK‑ iota
  Ser925-Val1065 (predicted)
  Accession # O75912
• Specificity: Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha ,
  -beta , -δ, -epsilon , -eta , -gamma , -kappa , -theta , or -zeta is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKI
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DGK-iota Antibody (648538)

• DAG kinase iota
• DGKI
• DGKiota
• DGK-iota
• diacylglycerol kinase iota
• diacylglycerol kinase, iota
• Diglyceride kinase iota
• EC 2.7.1.107

Background

Diacylglycerol kinase iota (DGK-iota ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-iota also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa 178-232 and aa 251-310), a catalytic domain (aa 372-507), and two ankyrin repeats (aa 958-990 and aa 997-1026). Over aa 925-1065, human DGK-iota shares 94% and 96% aa sequence identity with mouse and rat DGK-iota , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGK-iota Antibody (MAB6435)(1)

Publication using MAB6435

• VB Bartsch, JK Niehaus, B Taylor-Bla, MJ Zylka Enhanced histamine-induced itch in diacylglycerol kinase iota knockout mice PLoS ONE, 2019-06-05;14(6):e0217819. 2019-06-05 [PMID: 31167004] (IHC, Mouse)

Bioinformatics

• Gene Symbol: DGKI
• Uniprot:
  • O75912(Human)