HAF007). A specific band was detected for DGK-iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of SH-SY5Y human neuroblastoma cell line and mouse brain tissue. PVDF Membrane was probed with 1 µg/mL of Human/Mouse/Rat DGK-iota Antigen Affinity-purified Monoclonal Antibody (Catalog # MAB6435) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DGK-iota at approximately 115 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB6435-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP3-06558-50ul | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-85227-25ul | |||
![Immunohistochemistry-Paraffin: DGK-iota Antibody [NBP3-06558] - Staining of human DGKI in human hepatoma with rabbit polyclonal antibody at 1:100 dilution.](http://images.novusbio.com/fullsize/DGK-iota-Antibody-Immunohistochemistry-Paraffin-NBP3-06558-img0002.jpg "Immunohistochemistry-Paraffin: DGK-iota Antibody [NBP3-06558] - Staining of human DGKI in human hepatoma with rabbit polyclonal antibody at 1:100 dilution.")
![Immunocytochemistry/Immunofluorescence: DGK-iota Antibody [NBP3-06558] - Staining of DGKI in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with NBP3-06558 antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.](http://images.novusbio.com/fullsize/nbp3-06558_rabbit-polyclonal-dgk-iota-antibody-23520251628194.png)
![Immunocytochemistry/Immunofluorescence: DGK-iota Antibody [NBP1-85227] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.](http://images.novusbio.com/fullsize/DGK-iota-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-85227-img0004.jpg "Immunocytochemistry/Immunofluorescence: DGK-iota Antibody [NBP1-85227] - Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.")
