DGK-iota Antibody (648538) [mFluor Violet 500 SE]

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 648538
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: mFluor Violet 500 SE

DGK-iota Antibody (648538) [mFluor Violet 500 SE] Summary

• Immunogen: E. coli-derived recombinant human DGK‑ iota
  Ser925-Val1065 (predicted)
  Accession # O75912
• Specificity: Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha ,
  -beta , -δ, -epsilon , -eta , -gamma , -kappa , -theta , or -zeta is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGKI
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

mFluor(TM) is a trademark of AAT Bioquest, Inc. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DGK-iota Antibody (648538) [mFluor Violet 500 SE]

• DAG kinase iota
• DGKI
• DGKiota
• DGK-iota
• diacylglycerol kinase iota
• diacylglycerol kinase, iota
• Diglyceride kinase iota
• EC 2.7.1.107

Background

Diacylglycerol (DAG) influences numerous cell signaling cascades by functioning as an intracellular, allosteric activator of protein kinase C (PKC), and as a potent activator of guanine nucleotide exchange factors. In order to maintain cellular homeostasis, intracellular DAG levels are tightly regulated by diacylglycerol kinases (DGKs, DAGKs), which phosphorylate DAG to phosphatidic acid, thus removing DAG. Human DGK-alpha (80 kDa), -beta (90 kDa), and - gamma (90 kDa) have calcium-binding EF-hand motifs at their N termini and are classified as type I DGKs. Human DGK-delta (130 kDa) and DGK-

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGKI