DGK-iota Antibody (648538) [Alexa Fluor® 647]

• Applications: WB
• Clone: 648538
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 647

DGK-iota Antibody (648538) [Alexa Fluor® 647] Summary

• Immunogen: E. coli-derived recombinant human DGK‑ι
  Ser925-Val1065 (predicted)
  Accession # O75912
• Specificity: Detects human and mouse DGK‑ iota in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human DGK-alpha ,
  -beta , -δ, -epsilon , -eta , -gamma , -kappa , -theta , or -zeta is observed.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Diacylglycerol kinase iota (DGK-iota ) is an approximately 110 kDa kinase that phosphorylates the lipid diacylglycerol. DGK-iota also inhibits RasGRP3 and limits Rap1 activation. It contains two zinc finger regions (aa 178-232 and aa 251-310), a catalytic domain (aa 372-507), and two ankyrin repeats (aa 958-990 and aa 997-1026). Over aa 925-1065, human DGK-iota shares 94% and 96% aa sequence identity with mouse and rat DGK-iota , respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.