DGK-gamma Antibody

Images

 
Western Blot: DGK-gamma Antibody [NBP1-33055] - Sample (30 ug of whole cell lysate) A: H1299 7.5% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: DGK-gamma Antibody [NBP1-33055] - Analysis of methanol-fixed HeLa, using antibody at 1:200 dilution.
Immunohistochemistry-Paraffin: DGK-gamma Antibody [NBP1-33055] - Paraffin-embedded NCIN87 xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.92 mg/ml

Order Details

DGK-gamma Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DGK-gamma. The exact sequence is proprietary.
Localization
Cytoplasm, Membrane
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGKG
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 10 - 1:500
  • Immunohistochemistry-Paraffin 1:100-1:1000

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
0.92 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGK-gamma Antibody

  • DAG kinase gamma
  • DAGK3
  • DAGK3MGC104993
  • DGKG
  • DGKgamma
  • DGK-gamma
  • diacylglycerol kinase gamma
  • diacylglycerol kinase, gamma (90kD)
  • diacylglycerol kinase, gamma 90kDa
  • diacylglyerol kinase gamma
  • Diglyceride kinase gamma
  • EC 2.7.1.107
  • MGC133330

Background

This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DGKG
Entrez