DGAT2 Antibody - BSA Free

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Immunocytochemistry/ Immunofluorescence: DGAT2 Antibody [NBP3-05769] - NIH3T3 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Immunohistochemistry-Paraffin: DGAT2 Antibody [NBP3-05769] - Analysis of a FFPE tissue section of human liver using 1:200 dilution of DGAT2, catalog number NBP3-05769 antibody. The staining was developed using HRP ...read more
Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on NIH3T3 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more
Immunocytochemistry/ Immunofluorescence: DGAT2 Antibody [NBP3-05769] - HepG2 cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated ...read more
Flow (Intracellular): DGAT2 Antibody [NBP3-05769] - An intracellular stain was performed on HepG2 cells with DGAT2 Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

DGAT2 Antibody - BSA Free Summary

Immunogen
Partial recombinant protein made to an internal portion of the human DGAT2 protein (amino acids 268-377) [UniProt Q96PD7]
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Flow (Intracellular) 2.5 ug/ml
  • Flow Cytometry 2.5 ug/ml
  • Immunocytochemistry/Immunofluorescence 2 - 5 ug/ml
  • Immunohistochemistry 1:200
  • Immunohistochemistry-Paraffin 1:200
  • Western Blot 1 - 2 ug/ml

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGAT2 Antibody - BSA Free

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

Additional DGAT2 Products

Array NBP3-05769

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Diseases for DGAT2 Antibody (NBP3-05769)

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Blogs on DGAT2.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Bioinformatics

Gene Symbol DGAT2