DGAT1 Antibody

Product Discontinued

DGAT1 Antibody Summary

• Immunogen: Synthetic peptide, CYELNFPRSPRIRK, representing the internal region of the human protein according to NP_036211.1
• Epitope: CYELNFPRSPRIRK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DGAT1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:32000
  • Western Blot 0.3ug/ml
• Application Notes: Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 100kDa band in human adipose lysates after 0.3ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 55.3kDa according to NP_036211.1. The 100kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Reactivity Notes

Human, mouse, rat and dog.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris (pH 7.3), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Notes

Please note, this antibody is considered Innovators Grade. Innovators Grade antibodies are generally unvalidated and require additional characterization for most new species/applications. Novus has made these antibodies available through our Innovators Reward program. Complete an online review with image, detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Please contact us at innovators@novusbio.com for more details.

Alternate Names for DGAT1 Antibody

• ACAT-related gene product 1
• AGRP1
• ARAT
• ARGP1
• ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1
• DGAT
• DGAT1
• DGATACAT related gene product 1
• diacylglycerol O-acyltransferase 1
• diacylglycerol O-acyltransferase homolog 1 (mouse)
• diacylglycerol O-acyltransferase homolog 1
• DIAR7
• Diglyceride acyltransferase
• EC 2.3.1
• EC 2.3.1.20

Background

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DGAT1
• Entrez:
  • 8694(Human)
• Uniprot:
  • NP_036211.1(Human)