DFF45/ICAD Antibody (495403) [DyLight 550]

• Reactivity: Hu
• Applications: WB
• Clone: 495403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: DyLight 550

DFF45/ICAD Antibody (495403) [DyLight 550] Summary

• Immunogen: E. coli-derived recombinant human DFF45/ICAD
  Met1-Thr331 (predicted)
  Accession # O00273
• Specificity: Detects human DFF45/ICAD in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DFFA
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for DFF45/ICAD Antibody (495403) [DyLight 550]

• DFF1
• DFF1DNA fragmentation factor 45 kDa subunit
• DFF45
• DFF45DNA fragmentation factor subunit alpha
• DFF-45DNA fragmentation factor, 45 kD, alpha polypeptide
• DFFA
• DNA fragmentation factor, 45kDa, alpha polypeptide
• ICAD
• Inhibitor of CAD

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DFFA