DFF45/ICAD Antibody (495403) [Alexa Fluor® 488]

• Applications: WB
• Clone: 495403
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

DFF45/ICAD Antibody (495403) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human DFF45/ICAD
  Met1-Thr331 (predicted)
  Accession # O00273
• Specificity: Detects human DFF45/ICAD in direct ELISAs and Western blots.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

DFF45 (DNA fragmentation factor 45 kDa subunit, also known as ICAD) is a 45 kDa member of the CIDE domain-containing family of proteins. Human DFF45 is 331 amino acids (aa) in length. It contains an N-terminal CIDE domain (aa 17‑96) plus two caspase-3 cleavage sites (aa’s 117‑118 and 224‑225). DFF45 is a cytoplasmic protein that inhibits DFF40 DNAase. Normally, DFF45 and DFF40 form an enzymatically-inactive noncovalent heterodimer. Upon activation of the apoptotic cascade, DFF45 is cleaved into three fragments. These dissociate from DFF40 and induce DFF40 oligomerization and activation. Human DFF45 has two potential splice forms. One is truncated, and shows a seven aa substitution for aa 262‑331; a second is extended, and shows a 45 aa substitution for the C-terminal five amino acids. Human DFF45 is 76% aa identical to mouse DFF45.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.