Desmocollin-3 Antibody (765129)

Western blot shows lysates of mouse skin tissue and mouse embryo (15 d.p.c.) tissue. PVDF membrane was probed with 1 µg/mL of Rat Anti-Mouse Desmocollin-3 Monoclonal Antibody (Catalog # MAB7265) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for Desmocollin-3 at approximately 95-110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Desmocollin-3 was detected in perfusion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Anti-Mouse Desmocollin-3 Monoclonal Antibody (Catalog # MAB7265) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane and cytoplasm in epidermis. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clone: 765129
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Desmocollin-3 Antibody (765129) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Desmocollin-3
  Arg136-Leu695
  Accession # P55850
• Specificity: Detects mouse Desmocollin-3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 20% cross-reactivity with recombinant human Desmocollin-3 is observed and no cross-reactivity with recombinant mouse (rm) Desmocollin-1 or rmDesmocollin-2 is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: DSC3
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmocollin-3 Antibody (765129)

• Cadherin family member 3
• CDHF3
• CDHF3HT-CP
• desmocollin 3
• Desmocollin3
• Desmocollin-3
• desmocollin-4
• DSC
• DSC1
• DSC2
• DSC3
• DSC4
• DSC4desmocollin-3
• HT-CP

Background

DSC-3 (Desmocollin [Greek for "glue-that-binds"]-3) is a 95-110 kDa member of the Ca ++ -dependent cadherin family of adhesion molecules. It is found on the surface of stratified epithelial cells, including keratinized and nonkeratinized epithelia of the urinary bladder, vagina, oral cavity and skin. DSC-3 serves as a component of desmosomes, forming a linkage that unites adjacent cells with cytoplasmic intermediate filaments. In particular, in the extracellular space, DSC-3 forms both homotypic and heterotypic interactions with DSG-1 in-trans, and binds to the cytoskeleton intracellularly via plakophilin-3. Mature mouse DSC-3 is a 760 amino acid (aa) type I transmembrane glycoprotein. The mature molecule contains a 560 aa extracellular region with five cadherin domains (aa 136-691), and a 179 aa cytoplasmic domain that possesses three utilized Ser phosphorylation sites. There is one splice variant that shows an eight aa substitution for aa 831-895. Over aa 136-695, mouse DSC-3 shares 79% and 93% aa identity with human and rat DSC-3, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for Desmocollin-3 Antibody (MAB7265) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Qi Miao ICC Mouse 06/22/2017

Summary

• Application: Immunocytochemistry
• Sample Tested: skin keratinocytes
• Species: Mouse

Bioinformatics

• Gene Symbol: DSC3
• Uniprot:
  • P55850()