Desmin Antibody (OTI4G1)

Western Blot: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY Desmin (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-Desmin.

Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] Staining of paraffin-embedded Human prostate tissue using anti-Desmin mouse monoclonal antibody.

Flow Cytometry: Desmin Antibody (4G1) [NBP2-03539] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-Desmin antibody, and then analyzed by flow cytometry.

Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-Desmin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] - Staining of paraffin-embedded Human endometrium tissue using anti-Desmin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] - Staining of paraffin-embedded Human lymph node tissue using anti-Desmin mouse monoclonal antibody.

Immunohistochemistry-Paraffin: Desmin Antibody (4G1) [NBP2-03539] - Staining of paraffin-embedded Human lymphoma tissue using anti-Desmin mouse monoclonal antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC
• Clone: OTI4G1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.63 mg/ml

Desmin Antibody (OTI4G1) Summary

• Description: Novus Biologicals Mouse Desmin Antibody (OTI4G1) (NBP2-03539) is a monoclonal antibody validated for use in IHC, WB and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human fragment corresponding to amino acids 104-391 of human DES (NP_001918) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DES
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:100
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:200-500
• Theoretical MW: 53.4 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 0.63 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Desmin Antibody (OTI4G1)

• CMD1I
• CMD1IFLJ41013
• CSM1
• CSM2
• DES
• Desmin
• FLJ12025
• FLJ39719
• FLJ41793
• intermediate filament protein

Background

This gene encodes a muscle-specific class III intermediate filament. Homopolymers of this protein form a stable intracytoplasmic filamentous network connecting myofibrils to each other and to the plasma membrane. Mutations in this gene are associated with desmin-related myopathy, a familial cardiac and skeletal myopathy (CSM), and with distal myopathies. [provided by RefSeq].

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DES
• Entrez:
  • 1674(Human)