DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) PE.

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood using anti-CD148 (MEM-CD148/05) APC.

Flow Cytometry: DEP-1/CD148 Antibody (MEM-CD148/05) [NBP2-21790] - Surface staining of human peripheral blood leukocytes using anti-CD148 (MEM-CD148/05) biotin.

• Reactivity: Hu
• Applications: Flow, CyTOF-ready
• Clone: MEM-CD148/05
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free Summary

• Immunogen: Human recombinant CD148 (amino acids 1-444).
• Specificity: The mouse monoclonal antibody MEM-CD148/05 recognizes CD148, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRJ
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1-5 ug/ml
• Application Notes: This antibody is Cytof ready.
• Theoretical MW: 250 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for DEP-1/CD148 Antibody (MEM-CD148/05) - BSA Free

• CD148 antigen
• CD148
• Density-enhanced phosphatase 1
• DEP1
• DEP-1
• DEP1EC 3.1.3.48
• HPTP eta
• HPTPeta
• HPTP-eta
• human density enhanced phosphatase-1
• protein tyrosine phosphatase, receptor type, J polypeptide
• protein tyrosine phosphatase, receptor type, J
• Protein-tyrosine phosphatase eta
• Protein-tyrosine phosphatase receptor type J
• PTPRJ
• receptor-type tyrosine-protein phosphatase eta
• R-PTP-ETA
• R-PTP-J
• SCC1
• susceptibility to colon cancer 1, mouse, homolog of

Background

CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PTPRJ
• Entrez:
  • 5795(Human)