DEP-1/CD148 Antibody (A3)

CD148 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CD148 Monoclonal Antibody (Catalog #NBP3-45155) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the membrane of tonsil lymphocytes.

Human peripheral blood mononuclear cell (PBMC) lymphocytes were stained with Mouse Anti-Human CD148 Monoclonal Antibody (Catalog # NBP3-45155, filled histogram) or matched isotype control antibody (open histogram).

• Reactivity: Hu
• Applications: Flow, IHC
• Clone: A3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

DEP-1/CD148 Antibody (A3) Summary

• Description: Novus Biologicals Mouse DEP-1/CD148 Antibody (A3) (NBP3-45155) is a monoclonal antibody validated for use in IHC and Flow. Anti-DEP-1/CD148 Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: PHA-stimulated human PBMC
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: PTPRJ
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 2.0 ug/ml
  • Immunohistochemistry-Paraffin 1:100 - 1:500

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for DEP-1/CD148 Antibody (A3)

• CD148 antigen
• CD148
• Density-enhanced phosphatase 1
• DEP1
• DEP-1
• DEP1EC 3.1.3.48
• HPTP eta
• HPTPeta
• HPTP-eta
• human density enhanced phosphatase-1
• protein tyrosine phosphatase, receptor type, J polypeptide
• protein tyrosine phosphatase, receptor type, J
• Protein-tyrosine phosphatase eta
• Protein-tyrosine phosphatase receptor type J
• PTPRJ
• receptor-type tyrosine-protein phosphatase eta
• R-PTP-ETA
• R-PTP-J
• SCC1
• susceptibility to colon cancer 1, mouse, homolog of

Background

CD148 (also known as HPTP-eta or DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leucocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DEP-1/CD148 Antibody (NBP3-45155)(4)

Publications using NBP3-45155

• JE Baker, R Majeti, SG Tangye, et al. Protein tyrosine phosphatase CD148-mediated inhibition of T-cell receptor signal transduction is associated with reduced LAT and phospholipase Cgamma1 phosphorylation Mol Cell Biol. 2001-04-01 [PMID: 11259588]
• SG Tangye, YJ Liu, G Aversa, et al. Identification of functional human splenic memory B cells by expression of CD148 and CD27 J Exp Med. 1998-11-02 [PMID: 9802981]
• SG Tangye, J Wu, G Aversa, et al. Negative regulation of human T cell activation by the receptor-type protein tyrosine phosphatase CD148 J Immunol. 1998-10-15 [PMID: 9780142]
• SG Tangye, JH Phillips, LL Lanier, et al. CD148: a receptor-type protein tyrosine phosphatase involved in the regulation of human T cell activation J Immunol. 1998-10-01 [PMID: 9759839] (Flow Ctyometry, Human)

Bioinformatics

• Gene Symbol: PTPRJ