Human Defensin beta 1 ELISA Kit (Colorimetric)

ELISA: Human Defensin beta 1 ELISA Kit (Colorimetric) [NBP2-67933] - Samples were spiked with high concentrations of Human Defensin beta 1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Human Defensin beta 1 ELISA Kit (Colorimetric) [NBP2-67933] - Standard Curve Reference

• Reactivity: Hu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL

Human Defensin beta 1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Human DEFB1 in samples. No significant cross-reactivity or interference between Human DEFB1 and analogues was observed.
• Standard Curve Range: 31.25 - 2000 pg/mL
• Sensitivity: 18.75 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 5.12%
• Intra-Assay: CV% < 5.45%
• Spike Recovery: 87-104%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DEFB1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Human Defensin beta 1 ELISA Kit (Colorimetric)

• BD1
• BD-1
• beta-defensin 1
• beta-defensin-1
• DEFB-1
• DEFB101
• defensin, beta 1HBD-1
• hBD-1
• HBD1MGC51822

Background

Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Members of the defensin family arehighly similar in protein sequence. This gene encodes defensin, beta 1, an antimicrobial peptide implicated in theresistance of epithelial surfaces to microbial colonization. This gene maps in close proximity to defensin familymember, defensin, alpha 1 and has been implicated in the pathogenesis of cystic fibrosis. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for Defensin beta 1 ELISA Kit (NBP2-67933)(1)

Publication using NBP2-67933

• Radzey N, Harryparsad R, Meyer B et al. Genital inflammatory status and the innate immune response to contraceptive initiation American journal of reproductive immunology (New York, N.Y. : 1989) 2022-04-08 [PMID: 35394678] (ELISA)

FAQs for Defensin beta 1 ELISA Kit (NBP2-67933). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DEFB1