Defensin alpha 5 Antibody (8C8) [Alexa Fluor® 532]

• Reactivity: Hu
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC
• Clone: 8C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 532

Defensin alpha 5 Antibody (8C8) [Alexa Fluor® 532] Summary

• Immunogen: Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
• Localization: Secreted protein.
• Specificity: This is specific for HD5. It does not cross-react with HD6, lysozyme or sPLA2.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DEFA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

Alexa Fluor (R) products are provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Defensin alpha 5 Antibody (8C8) [Alexa Fluor® 532]

• alpha-Defensin 5
• DEF5
• DEF5defensin, alpha 5, preproprotein
• DEFA5
• defensin 5
• Defensin alpha 5
• Defensin, alpha 5
• defensin, alpha 5, Paneth cell-specific
• defensin-5
• HD-5
• MGC129728

Background

Defensin alpha 5 (DEFA5) is primarily responsible for host defense. DEFA5 has shown antimicrobial activity and is thought to kill microbes by permeabilizing their plasma membrane. This protein is highly expressed in the secretory granules of Paneth cells of the ileum.

Defensin alpha 5 antibodies are useful tools for bacterial defense studies and human immunology research.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DEFA5