Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

Immunohistochemistry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693] - Human skeletal muscle myocytes showing moderate nuclear and faint cytoplasmic staining. Image from the standard format of this antibody.

Flow Cytometry: Defensin alpha 5 Antibody (8C8) - Azide and BSA Free [NBP2-80693] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Defensin alpha 5 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used. Image from the standard format of this antibody.

• Reactivity: Hu
• Applications: WB, DB, ELISA, Flow, ICC/IF, IHC
• Clone: 8C8
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 2.35 mg/ml

Defensin alpha 5 Antibody (8C8) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse Defensin alpha 5 Antibody (8C8) - Azide and BSA Free (NB110-60002) is a monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full-length human Defensin alpha 5 [Swiss-Prot# Q01523]. The propeptide is the full-length protein. In the mature form, it is cleaved at residue 63 (alanine).
• Localization: Secreted protein.
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: DEFA5
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot reported in scientific literature
  • ELISA reported in scientific literature
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 5-10 ug/ml
  • Immunohistochemistry-Frozen reported in scientific literature (PMID 30612153)
  • Immunohistochemistry-Paraffin 5-10 ug/ml
  • Western Blot 1:1000
• Application Notes: In Western blot a band is observed ~10 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: Tris-Glycine, 0.15 M NaCl
• Preservative: No Preservative
• Concentration: 2.35 mg/ml
• Purity: Protein G purified

Alternate Names for Defensin alpha 5 Antibody (8C8) - Azide and BSA Free

• alpha-Defensin 5
• DEF5
• DEF5defensin, alpha 5, preproprotein
• DEFA5
• defensin 5
• Defensin alpha 5
• Defensin, alpha 5
• defensin, alpha 5, Paneth cell-specific
• defensin-5
• HD-5
• MGC129728

Background

Defensins are a kind of endogenous antimicrobial peptide enriched with cysteine/arginine and are widely distributed in internal organs wherein they are recognized as defenders against invasion by pathogenic organisms. Human alpha-defensins (human alpha-defensins 5, -6, and human neutrophil peptides-1, -2, -3, -4) are a class of small antimicrobial peptides with well characterized antibacterial activity against both Gram +ve as well as Gram-ve bacteria, and certain viruses such as such as HSV, HPV, adenovirus and polyoma virus. Human alpha-defensin 5 (HD5) is a 32 amino acids long protein that shares a common structural feature of alpha-defensin family characterized by three-stranded anti-parallel beta-sheets stabilized by three intra-molecular disulfide bridges. HD5 is primarily expressed in small intestinal Paneth cells or epithelial cells of urino-genital tract, and it is found in cytoplasmic vesicle/secretory vesicle or in secreted form. Peptides HD5(20-94), HD5(23-94) and HD5(29-94) are found within tissues among which HD5(20-94) is the predominant intracellular form. In extracellular milieu, peptides HD5(56-94) and HD5(63-94) are found and among these two peptides, HD5 (63-94) is the most abundant form.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DEFA5