![Western Blot: DEF6 Antibody [NBP1-81618] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.](http://images.novusbio.com/fullsize/DEF6-Antibody-Western-Blot-NBP1-81618-img0012.jpg "Western Blot: DEF6 Antibody [NBP1-81618] - Analysis in human cell lines A-431 and A-549. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.")
| Reactivity | Hu, Rt, MuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Description | Novus Biologicals Rabbit DEF6 Antibody - BSA Free (NBP1-81618) is a polyclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee. |
| Immunogen | This antibody was developed against Recombinant Protein corresponding to amino acids: RADEDVEAAQRKLRQASTNVKHWNVQMNRLMHPIEPGDKRPVTSSSFSGFQPPLLAHRDSSLKRLTRWGSQGNRTPSPNSNEQQKSLNGG |
| Predicted Species | Mouse (91%). Backed by our 100% Guarantee. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DEF6 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | For IHC-Paraffin, HIER pH 6 retrieval is recommended. |
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| Control Peptide |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.2) and 40% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Purity | Immunogen affinity purified |
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Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | DEF6 |
![Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining in human lymph node and kidney tissues using anti-DEF6 antibody. Corresponding DEF6 RNA-seq data are presented for the same tissues.](http://images.novusbio.com/fullsize/DEF6-Antibody-Immunohistochemistry-Paraffin-NBP1-81618-img0011.jpg "Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining in human lymph node and kidney tissues using anti-DEF6 antibody. Corresponding DEF6 RNA-seq data are presented for the same tissues.")
![Western Blot: DEF6 Antibody [NBP1-81618] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4](http://images.novusbio.com/fullsize/DEF6-Antibody-Western-Blot-NBP1-81618-img0005.jpg "Western Blot: DEF6 Antibody [NBP1-81618] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4")
![Western Blot: DEF6 Antibody [NBP1-81618] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.](http://images.novusbio.com/fullsize/DEF6-Antibody-Western-Blot-NBP1-81618-img0013.jpg "Western Blot: DEF6 Antibody [NBP1-81618] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.")
![Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining of human kidney shows low expression as expected.](http://images.novusbio.com/fullsize/DEF6-Antibody-Immunohistochemistry-Paraffin-NBP1-81618-img0009.jpg "Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining of human kidney shows low expression as expected.")
![Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining of human lymph node shows high expression.](http://images.novusbio.com/fullsize/DEF6-Antibody-Immunohistochemistry-Paraffin-NBP1-81618-img0010.jpg "Immunohistochemistry-Paraffin: DEF6 Antibody [NBP1-81618] - Staining of human lymph node shows high expression.")
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")