Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

Western Blot: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Analysis of Dectin-2 in human heart lysate in the 1) absence and 2) presence of immunizing peptide, 3) mouse heart lysate, 4) rat heart lysate and 5) human Ramos cell lysate using Dectin-2 antibody at 2 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test. Image from the standard format of this antibody.

Immunohistochemistry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Normal human spleen tissue stained with Dectin-2 antibody (5 ug/mL), peroxidase-conjugate and DAB chromogen. Human tissue TMA was used for this test. Image from the standard format of this antibody.

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - An intracellular stain was performed on THP-1 cells with Dectin-2/CLEC6A (3D1) antibody NBP2-27159AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were directly conjugated to Alexa Fluor 488.

Flow Cytometry: Dectin-2/CLEC6A Antibody (3D1) - Azide Free [NBP2-36486] - Surface staining of Dectin-2 on 5x10^5 human PBMCs using 0.5 ug of Dectin-2 antibody (red) and 0.5 ug of isotype control antibody (green). Image from the standard format of this antibody.

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 3D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free Summary

• Immunogen: A portion of amino acids 75-139 of human Dectin-2 was used as the immunogen for the antibody. 90-106
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC6A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1ul/1 million cells
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 5 ug/ml
  • Western Blot 2-5 ug/ml

Reactivity Notes

Orangutan, Chimpanzee (100%), Rhesus monkey, Bovine, Sheep (83%), Mouse (65%), Rat (52%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Dectin-2/CLEC6A Antibody (3D1) - Azide and BSA Free

• CLEC4N
• CLEC6A
• CLECSF10
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
• C-type lectin domain family 6 member A
• C-type lectin domain family 6, member A
• C-type lectin superfamily member 10
• DC-associated C-type lectin 2
• dectin 2
• Dectin2
• Dectin-2
• Dendritic cell-associated C-type lectin 2

Background

Dectin-2 is a C-type lectin expressed by dendritic cells, macrophages and inflammatory monocytes. It is a type II membrane receptor, with a short cytoplasmic tail, and a C-terminal C-type lectin domain. Dectin-2 is a receptor with specificity for high mannose structures and mediates cytokine production from DC and other antigen presenting cells. Though, Dectin-2 has no obvious signaling motifs attached to CP tail, reports indicate that Dectin-2 employs FcR 7; for internalization and to activate NF-kB pathway for cytokine production. Though it shares only 20-25% of homology with Dectin-1, it functions similarly as pattern recognition receptor (PRR) for fungal infections, to activate both innate (Syk-CARD9 innate signaling pathway) and adaptive immune responses (by activation of DC) to fungal infections. Thus Dectin-2 is a connector molecule of both innate and adaptive immunity functioning at DC level.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CLEC6A