Dectin-2/CLEC6A Antibody (3D1) [PE]

Flow Cytometry: Mouse Monoclonal Dectin-2/CLEC6A Antibody (3D1) [PE] [NBP2-27159PE] - Dectin-2/CLEC6A expression on human monocyte-derived macrophages. Macrophages were treated with 10 μg/ml of the Dectin-2/CLEC6A Antibody (3D1) [PE] (catalog # NBP2-27159PE) (green) or mouse IgG3 PE isotype control antibody (red) for 30 minutes on ice. Image from a verified customer review.

Flow Cytometry: Mouse Monoclonal Dectin-2/CLEC6A Antibody (3D1) [PE] [NBP2-27159PE] - Dectin-2/CLEC6A expression on human monocyte-derived dendritic cells. DCs were treated with 10 ug/ml of the Dectin-2/CLEC6A antibody (blue) or mouse IgG3 PE isotype control antibody (red) for 30 minutes on ice. Image from a verified customer review.

• Reactivity: Hu, Pm
• Applications: WB, Flow, IHC, CyTOF-ready
• Clone: 3D1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PE

Dectin-2/CLEC6A Antibody (3D1) [PE] Summary

• Immunogen: A portion of amino acids 75-139 of human Dectin-2 was used as the immunogen for the antibody. 90-106
• Isotype: IgG3 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: CLEC6A
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Bovine, Sheep (83%), Mouse (65%), Rat (52%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Dectin-2/CLEC6A Antibody (3D1) [PE]

• CLEC4N
• CLEC6A
• CLECSF10
• C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamilymember 10
• C-type lectin domain family 6 member A
• C-type lectin domain family 6, member A
• C-type lectin superfamily member 10
• DC-associated C-type lectin 2
• dectin 2
• Dectin2
• Dectin-2
• Dendritic cell-associated C-type lectin 2

Background

Dectin 2 is a type II transmembrane protein that is a member of the C type lectin superfamily. In mouse, dectin 2 is predominantly expressed on tissue macrophages and some dendritic cells. Significant expression of Dectin 2 has been reported on macrophages in the red pulp and marginal zones of the spleen, kupffer cells in the liver and alveolar macrophages in the lung. Peripheral blood monocytes express low levels of dectin 2 but transient up regulation of expression has been demonstrated on monocytes at sites of inflammation. The function of dectin 2 has not been fully determined but studies suggest a possible role in immune surveillance.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Reviews for Dectin-2/CLEC6A Antibody (NBP2-27159PE) (2)

Average Rating: 5

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/29/2025

Summary

• Application: Flow Cytometry
• Sample Tested: Monocyte-derived dendritic cells
• Species: Human
• Lot: D169169

reviewed by: Panagiotis Fotakis Flow Human 08/13/2024

Summary

• Application: Flow Cytometry
• Sample Tested: monocyte-derived macrophages
• Species: Human
• Lot: D167086

Bioinformatics

• Gene Symbol: CLEC6A