Decorin Antibodies: Novus Biologicals

AF1060

Decorin Antibody [Unconjugated]

(1 Review)

100 μg

AF1060

HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse adipose tissue and mouse colon tissue. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Decorin at approximately 65-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_lightbox">

Simple Western lane view shows lysates of mouse adipose tissue and mouse colon tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 94 kDa (as indicated) using 10 µg/mL of Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # <a class=

HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system." class="big_thumb" />

Increased CSF-decorin in three different mouse models of A beta pathology. a, b Immunostaining and quantification of decorin in the ChP. Scale bars, 100 µm. (n = 4). c Double immunostaining of DCN and PV or d SRIF in mouse hippocampus. e Quantification of decorin-positive cell-type distribution. Scale bars, 500 µm. (n = 3). f, g Immunostaining and quantification of decorin in the hippocampus. Scale bars, 500 µm. (n = 4). h Mouse CSF-decorin levels in three months old (n = 5), i 13 months old (n = 4–5), j 18 months old (n = 3–5) mice were measured by ELISA and quantified. k CSF-decorin levels in AppNL-F/NL-F mice of different ages were measured and quantified. (n = 3–5). l Decorin levels in A beta 42 treated mouse primary neurons and (m) were quantified (n = 4). n Quantification of decorin levels in conditioned media. (n = 8). Data in (b, g–k) were analyzed by one-way ANOVA followed by Dunnett’s multiple comparisons test. Data in (m, n) were analyzed by student’s t-test. Data in (e) were analyzed by two-way ANOVA followed by Tukey’s post hoc test. Data are represented as mean ± SEM; *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. DCN decorin, PV parvalbumin, SRIF somatotropin release-inhibiting factor, PN pyramidal neurons, ns not significant Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/35787306), licensed under a CC-BY license. Not internally tested by R&D Systems.

1060-DE) was serially diluted and captured by Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse Decorin (Catalog # <a class=

1060-DE) was serially diluted and captured by Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1060-DE) was serially diluted and captured by Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

1060-DE) was serially diluted and captured by Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1060) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

, , ,

Applications: , , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF1060U-100UG
Alexa Fluor 405	AF1060V-100UG
Alexa Fluor 488	AF1060G-100UG
Alexa Fluor 532	AF1060X-100UG
Alexa Fluor 594	AF1060T-100UG
Alexa Fluor 647	AF1060R-100UG
Alexa Fluor 700	AF1060N-100UG
Alexa Fluor 750	AF1060S-100UG
Alexa Fluor Plus 405	AF1060AFP405-100UG
Alexa Fluor Plus 488	AF1060AFP488-100UG
Alexa Fluor Plus 555	AF1060AFP555-100UG
Alexa Fluor Plus 594	AF1060AFP594-100UG
Alexa Fluor Plus 647	AF1060AFP647-100UG
Alexa Fluor Plus 680	AF1060AFP680-100UG
Allophycocyanin	AF1060A
Biotin	BAF1060
CoraFluor 1	AF1060CL1
DyLight 350	AF1060D
DyLight 405	AF1060E
DyLight 488	AF1060K
DyLight 550	AF1060L
DyLight 594	AF1060M
DyLight 650	AF1060W
DyLight 680	AF1060Y
DyLight 755	AF1060Z
FITC	AF1060F
HRP	AF1060H
Janelia Fluor 525	AF1060JF525
Janelia Fluor 549	AF1060I
Janelia Fluor 646	AF1060J
Janelia Fluor 669	AF1060JF669
PE	AF1060P
PerCP	AF1060C
mFluor Violet 450 SE	AF1060MFV450
mFluor Violet 500 SE	AF1060MFV500
mFluor Violet 610 SE	AF1060MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF1060-SP

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AF143

Decorin Antibody [Unconjugated]

(31 Publications)

100 μg

AF143

HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (<a class=

HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF017). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Decorin was detected in immersion fixed paraffin-embedded sections of human breast using Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; (<a class=

CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to intralobular stromal tissue. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Simple Western lane view shows lysates of human uterus and human heart tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 140-149 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Decorin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF143) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemical DCN expression in HSc and site-matched NS from burn patients.(a) Immunohistochemistry using a polyclonal goat anti-human DCN antibody and Alexa Fluor 488 secondary antibody (green fluorescence), and counterstained with DAPI (blue fluorescence) in representative site-matched sections of NS and HSc (scale bar = 50 μm). (b) Relative expression of DCN in matched superficial and deep NS and HSc sections was calculated from fluorescence using ImageJ (mean ± SEM, n = 4 patients, * P < 0.001). Image collected and cropped by CiteAb from the following open publication (//dx.plos.org/10.1371/journal.pone.0123054), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF143U-100UG
Alexa Fluor 405	AF143V-100UG
Alexa Fluor 488	AF143G-100UG
Alexa Fluor 532	AF143X-100UG
Alexa Fluor 594	AF143T-100UG
Alexa Fluor 647	AF143R-100UG
Alexa Fluor 700	AF143N-100UG
Alexa Fluor 750	AF143S-100UG
Alexa Fluor Plus 405	AF143AFP405-100UG
Alexa Fluor Plus 488	AF143AFP488-100UG
Alexa Fluor Plus 555	AF143AFP555-100UG
Alexa Fluor Plus 594	AF143AFP594-100UG
Alexa Fluor Plus 647	AF143AFP647-100UG
Alexa Fluor Plus 680	AF143AFP680-100UG
Allophycocyanin	AF143A
Biotin	BAF143
CoraFluor 1	AF143CL1
DyLight 350	AF143D
DyLight 405	AF143E
DyLight 488	AF143K
DyLight 550	AF143L
DyLight 594	AF143M
DyLight 650	AF143W
DyLight 680	AF143Y
DyLight 755	AF143Z
FITC	AF143F
HRP	AF143H
Janelia Fluor 525	AF143JF525
Janelia Fluor 549	AF143I
Janelia Fluor 646	AF143J
Janelia Fluor 669	AF143JF669
PE	AF143P
PerCP	AF143C
mFluor Violet 450 SE	AF143MFV450
mFluor Violet 500 SE	AF143MFV500
mFluor Violet 610 SE	AF143MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF143-SP

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MAB143

Decorin Antibody (115402) [Unconjugated]

(2 Reviews)

(18 Publications)

100 μg

MAB143

HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox">

Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human uterus tissue and human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

Simple Western lane view shows lysates of human uterus tissue, loaded at 0.2 mg/mL. A specific band was detected for Decorin at approximately 145 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB143) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Immunohistochemical staining of stromal protein expression in DCIS with sclerotic or myxoid stromaMicrophotographs displaying HE staining (A-B), and IHC staining for biglycan (C-D), decorin (E-F) and versican (G-H). Panels A-C-E-G display photographs of one DCIS lesion with sclerotic stroma; panels B-D-F-H display one DCIS lesion with myxoid stroma. This figure illustrates that myxoid DCIS present reduced periductal decorin staining and tend to have increased periductal versican and biglycan expression, whereas sclerotic DCIS generally present strong stromal decorin immunoreactivity, and tend to lack stromal versican and biglycan. Original magnification 100x. Image collected and cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by R&D Systems.

<b>Biological Strategies Validation. </b>Treatment of CAFs with combined cytokines or cancer cell-derived secretomes affects ECM protein expression(A) Western blot showing the effects of bFGF, EGF and TGF-beta 1 treatment (10 ng/ml) on the expression of biglycan, decorin, alpha -SMA and versican in CAFs. (B) Western blot illustrating the effects of treatment with cancer cell-derived conditioned medium (CM) on the expression of biglycan, decorin, alpha -SMA and versican in CAFs. TGF-beta 1 treatment (1 and 10 ng/ml) was included as a positive control. Tubulin was used as loading control (A, B). Image collected and cropped by CiteAb from the following publication (//www.oncoscience.us/lookup/doi/10.18632/oncoscience.87), licensed under a CC-BY license. Not internally tested by R&D Systems.

Secretome analysis of WT, COG4p.G516R, and COG4-KO chondrosarcoma cells. (A) Coomassie blue staining of proteins in cell lysates and conditioned medium from COG4p.G516R clones #1, #2, WT controls and COG4 KO cells. (B) Volcano plot of differentially secreted proteins between COG4p.G516R and WT. (C) Volcano plot of differentially secreted proteins between COG4p.G516R and COG4-KO cells. A few top candidates confirmed by pathway analysis were labeled respectively. (D) Dot plot showing enriched pathways from Gene Set Enrichment Analysis with the Normalized Enrichment Score (NES) shown on the X-axis. The size of the dots represents the number of genes in the significant Data Set list associated with the GO term and the color of the dots represent the adjusted p- values. (E) Table showing a list of top 10 candidates which were decreased in secretions of COG4p.G516R with log 2 (Fold Change) and adjusted p-value shown for COG4p.G516R vs. WT and COG4p.G516R vs. COG4-KO. The number of peptides is equal to or more than two for all the candidates shown in table. (F) Western blotting of top candidates in medium decreased in both COG4p.G516R and COG4-KO cells compared to WT. GAPDH was from cell lysates with comparable amount as loading control. (G) Western blotting of top candidates only changed in COG4p.G516R compared to WT and COG4-KO cells. All experiments were performed in duplicate with similar results. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/36393834), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB143U-100UG
Alexa Fluor 405	FAB143V-100UG
Alexa Fluor 488	FAB143G-100UG
Alexa Fluor 532	FAB143X-100UG
Alexa Fluor 594	FAB143T-100UG
Alexa Fluor 647	FAB143R-100UG
Alexa Fluor 700	FAB143N-100UG
Alexa Fluor 750	FAB143S-100UG
Alexa Fluor Plus 405	FAB143AFP405-100UG
Alexa Fluor Plus 488	FAB143AFP488-100UG
Alexa Fluor Plus 555	FAB143AFP555-100UG
Alexa Fluor Plus 594	FAB143AFP594-100UG
Alexa Fluor Plus 647	FAB143AFP647-100UG
Alexa Fluor Plus 680	FAB143AFP680-100UG
Allophycocyanin	FAB143A
Biotin	FAB143B
CoraFluor 1	FAB143CL1
DyLight 350	FAB143D
DyLight 405	FAB143E
DyLight 488	FAB143K
DyLight 550	FAB143L
DyLight 594	FAB143M
DyLight 650	FAB143W
DyLight 680	FAB143Y
DyLight 755	FAB143Z
FITC	FAB143F
HRP	FAB143H
Janelia Fluor 525	FAB143JF525
Janelia Fluor 549	FAB143I
Janelia Fluor 646	FAB143J
Janelia Fluor 669	FAB143JF669
PE	FAB143P
PerCP	FAB143C
mFluor Violet 450 SE	FAB143MFV450
mFluor Violet 500 SE	FAB143MFV500
mFluor Violet 610 SE	FAB143MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB143-SP
Carrier Free	MAB143-500

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NBP3-20641

Decorin Antibody (DCN/6289)

100 μg

NBP3-20641

Formalin-fixed, paraffin-embedded human prostate stained with Decorin antibody (DCN/6289). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Formalin-fixed, paraffin-embedded human placenta stained with Decorin antibody (DCN/6289). Inset: PBS instead of primary antibody; secondary only negative control.

Reactivity:

Hu, Mu, Po, Bv, Ca, Ch, Eq, Ma-CC, Rb

Species Glossary

Reactivity: Hu, Mu, Po, Bv, Ca, Ch, Eq, Ma-CC, Rb

Applications:

, , ,

Applications: , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-21077AF350
Alexa Fluor 405	NBP3-21077AF405
Alexa Fluor 488	NBP3-21077AF488
Alexa Fluor 532	NBP3-21077AF532
Alexa Fluor 594	NBP3-21077AF594
Alexa Fluor 647	NBP3-21077AF647
Alexa Fluor 700	NBP3-21077AF700
Alexa Fluor 750	NBP3-21077AF750
Alexa Fluor Plus 405	NBP3-21077AFP405
Alexa Fluor Plus 488	NBP3-21077AFP488
Alexa Fluor Plus 555	NBP3-21077AFP555
Alexa Fluor Plus 594	NBP3-21077AFP594
Alexa Fluor Plus 647	NBP3-21077AFP647
Alexa Fluor Plus 680	NBP3-21077AFP680
Allophycocyanin	NBP3-21077APC
Biotin	NBP3-21077B
CoraFluor 1	NBP3-21077CL1
DyLight 350	NBP3-21077UV
DyLight 405	NBP3-21077V
DyLight 488	NBP3-21077G
DyLight 550	NBP3-21077R
DyLight 594	NBP3-21077DL594
DyLight 650	NBP3-21077C
DyLight 680	NBP3-21077FR
DyLight 755	NBP3-21077IR
FITC	NBP3-21077F
HRP	NBP3-21077H
Janelia Fluor 525	NBP3-21077JF525
Janelia Fluor 549	NBP3-21077JF549
Janelia Fluor 646	NBP3-21077JF646
Janelia Fluor 669	NBP3-21077JF669
PE	NBP3-21077PE
PerCP	NBP3-21077PCP
mFluor Violet 450 SE	NBP3-21077MFV450
mFluor Violet 500 SE	NBP3-21077MFV500
mFluor Violet 610 SE	NBP3-21077MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP3-21077

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NBP1-84970

Decorin Antibody - BSA Free

(7 Publications)

0.1 ml

NBP1-84970

Western Blot: Decorin Antibody [NBP1-84970] - Analysis in human cell line U-2197.

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining of human cerebral cortex shows low expression as expected.

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-84970] - Staining of human ovary shows high expression.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Validated by:

Validated by:

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NBP1-57923

Decorin Antibody - BSA Free

(1 Review)

(1 Publication)

100 ul

NBP1-57923

Immunocytochemistry/Immunofluorescence: Decorin Antibody [NBP1-57923] - Human periodontal ligament cells stained with rabbit anti-decorin 1:300 and goat anti-rabbit Alexa 488 1:500. Nuclei stained with DAPI. ICC/IF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: Decorin Antibody [NBP1-57923] - Human skin tissue at an antibody concentration of 4-8 ug/mL.

Immunohistochemistry: Decorin Antibody [NBP1-57923] - Comparative immunohistochemical analysis of non-chondrodystrophic (NCD) & chondrodystrophic (CD) canine nucleus pulposus (NP) expression & distribution of decorin, biglycan, fibromodulin, hyaluronan & proteoglycan link protein 1 (HAPLN1), & aggrecan. For all extracellular matrix (ECM) proteins, the NCD canine intervertebral disc NP reveals a cobweb appearance, demonstrating intense staining for all proteins located in the areas tightly between the cells. Immunostaining with decorin reveals diffuse intercellular staining with negative immunostaining within the large, physaliferous-appearing notochordal cells. This presentation is in contrast to the abundant staining of these proteins in every CD NP sample. Decorin, biglycan, & HAPLN1 reveal intense staining within the ECM, with abundant clusters of small numbers of cells present within the NP. Although the ECM staining is less intense than that for the other three proteins, fibromodulin & aggrecan immunostaining is present. Furthermore, the CD NP stained for aggrecan reveals intense pericellular immunostaining diffusely throughout the ECM that is much less cellular than the NCD NP staining. Safranin-O staining shows quite intense ECM staining in the CD NP, whereas the NCD sample demonstrates intense intercellular staining without large, acellular ECM areas rich in proteoglycan staining. The overall appearance of the CD NP bears a strong resemblance to a fibrocartilaginous phenotype that is distinctly different from the NCD canine NP Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/26341258), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Reactivity:

Hu, Ca

Species Glossary

Reactivity: Hu, Ca

Applications:

, ,

Applications: , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

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NBP2-79774

Decorin Antibody (DCN/3523)

100 μg

NBP2-79774

Protein Array: Decorin Antibody (DCN/3523) [NBP2-79774] - Analysis of Protein Array containing more than 19,000 full-length human proteins using Decorin Antibody (DCN/3523) to Decorin (DCN/3523). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP2-79908AF350
Alexa Fluor 405	NBP2-79908AF405
Alexa Fluor 488	NBP2-79908AF488
Alexa Fluor 532	NBP2-79908AF532
Alexa Fluor 594	NBP2-79908AF594
Alexa Fluor 647	NBP2-79908AF647
Alexa Fluor 700	NBP2-79908AF700
Alexa Fluor 750	NBP2-79908AF750
Alexa Fluor Plus 405	NBP2-79908AFP405
Alexa Fluor Plus 488	NBP2-79908AFP488
Alexa Fluor Plus 555	NBP2-79908AFP555
Alexa Fluor Plus 594	NBP2-79908AFP594
Alexa Fluor Plus 647	NBP2-79908AFP647
Alexa Fluor Plus 680	NBP2-79908AFP680
Allophycocyanin	NBP2-79908APC
Biotin	NBP2-79908B
CoraFluor 1	NBP2-79908CL1
DyLight 350	NBP2-79908UV
DyLight 405	NBP2-79908V
DyLight 488	NBP2-79908G
DyLight 550	NBP2-79908R
DyLight 594	NBP2-79908DL594
DyLight 650	NBP2-79908C
DyLight 680	NBP2-79908FR
DyLight 755	NBP2-79908IR
FITC	NBP2-79908F
HRP	NBP2-79908H
Janelia Fluor 525	NBP2-79908JF525
Janelia Fluor 549	NBP2-79908JF549
Janelia Fluor 646	NBP2-79908JF646
Janelia Fluor 669	NBP2-79908JF669
PE	NBP2-79908PE
PerCP	NBP2-79908PCP
mFluor Violet 450 SE	NBP2-79908MFV450
mFluor Violet 500 SE	NBP2-79908MFV500
mFluor Violet 610 SE	NBP2-79908MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Azide and BSA Free	NBP2-79908

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NBP3-35694

Decorin Antibody - BSA Free

100 ul

NBP3-35694

Immunocytochemistry/ Immunofluorescence: Decorin Antibody [NBP3-35694] - Immunofluorescence analysis of MCF-7 cells using Decorin Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: Decorin Antibody [NBP3-35694] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Decorin Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: Decorin Antibody [NBP3-35694] - Immunohistochemistry analysis of paraffin-embedded Human colon using Decorin Rabbit pAb at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Reactivity:

Hu, Mu, Rt

Species Glossary

Reactivity: Hu, Mu, Rt

Applications:

, , ,

Applications: , , ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Conjugate:

Unconjugated

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP3-35694-20ul

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MAB1432

Decorin Antibody (115424)

(2 Publications)

500 μg

MAB1432

143-DE) was serially diluted and captured by Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB1432) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Decorin Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_lightbox">

Recombinant Human Decorin (Catalog # <a class=

143-DE) was serially diluted and captured by Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB1432) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Decorin Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" alt="Recombinant Human Decorin (Catalog # 143-DE) was serially diluted and captured by Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB1432) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human Decorin Biotinylated Monoclonal Antibody (Catalog # [[catalogNumber:) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # [[catalogNumber:DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1432U-100UG
Alexa Fluor 405	FAB1432V-100UG
Alexa Fluor 488	FAB1432G-100UG
Alexa Fluor 532	FAB1432X-100UG
Alexa Fluor 594	FAB1432T-100UG
Alexa Fluor 647	FAB1432R-100UG
Alexa Fluor 700	FAB1432N-100UG
Alexa Fluor 750	FAB1432S-100UG
Alexa Fluor Plus 405	FAB1432AFP405-100UG
Alexa Fluor Plus 488	FAB1432AFP488-100UG
Alexa Fluor Plus 555	FAB1432AFP555-100UG
Alexa Fluor Plus 594	FAB1432AFP594-100UG
Alexa Fluor Plus 647	FAB1432AFP647-100UG
Alexa Fluor Plus 680	FAB1432AFP680-100UG
Allophycocyanin	FAB1432A
Biotin	FAB1432B
CoraFluor 1	FAB1432CL1
DyLight 350	FAB1432D
DyLight 405	FAB1432E
DyLight 488	FAB1432K
DyLight 550	FAB1432L
DyLight 594	FAB1432M
DyLight 650	FAB1432W
DyLight 680	FAB1432Y
DyLight 755	FAB1432Z
FITC	FAB1432F
HRP	FAB1432H
Janelia Fluor 525	FAB1432JF525
Janelia Fluor 549	FAB1432I
Janelia Fluor 646	FAB1432J
Janelia Fluor 669	FAB1432JF669
PE	FAB1432P
PerCP	FAB1432C
mFluor Violet 450 SE	FAB1432MFV450
mFluor Violet 500 SE	FAB1432MFV500
mFluor Violet 610 SE	FAB1432MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1432-SP

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MAB11528

Decorin Antibody (1072716)

100 μg

MAB11528

HAF018). A specific band was detected for Decorin at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox">

Western Blot shows lysates of human uterus. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB11528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # <a class=

HAF018). A specific band was detected for Decorin at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." alt="Western Blot shows lysates of human uterus. PVDF membrane was probed with 1 µg/ml of Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB11528) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Decorin at approximately 80-100 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1." class="big_thumb" />

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of stroma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

Decorin was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Decorin Monoclonal Antibody (Catalog # MAB11528) at 5 µg/ml for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # <a class=

VC001) or the HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of stroma cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Allophycocyanin	FAB11528A
Biotin	FAB11528B
CoraFluor 1	FAB11528CL1
DyLight 350	FAB11528D
DyLight 405	FAB11528E
DyLight 488	FAB11528K
DyLight 550	FAB11528L
DyLight 594	FAB11528M
DyLight 650	FAB11528W
DyLight 680	FAB11528Y
DyLight 755	FAB11528Z
FITC	FAB11528F
HRP	FAB11528H
Janelia Fluor 525	FAB11528JF525
Janelia Fluor 549	FAB11528I
Janelia Fluor 646	FAB11528J
Janelia Fluor 669	FAB11528JF669
PE	FAB11528P
PerCP	FAB11528C
mFluor Violet 450 SE	FAB11528MFV450
mFluor Violet 500 SE	FAB11528MFV500
mFluor Violet 610 SE	FAB11528MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB11528-SP

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MAB1060

Decorin Antibody (161026)

(2 Publications)

500 μg

Reactivity:

Mu

Species Glossary

Reactivity: Mu

Applications:

Applications:

Host:

Rat Monoclonal

Host: Rat Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB1060U-100UG
Alexa Fluor 405	FAB1060V-100UG
Alexa Fluor 488	FAB1060G-100UG
Alexa Fluor 532	FAB1060X-100UG
Alexa Fluor 594	FAB1060T-100UG
Alexa Fluor 647	FAB1060R-100UG
Alexa Fluor 700	FAB1060N-100UG
Alexa Fluor 750	FAB1060S-100UG
Alexa Fluor Plus 405	FAB1060AFP405-100UG
Alexa Fluor Plus 488	FAB1060AFP488-100UG
Alexa Fluor Plus 555	FAB1060AFP555-100UG
Alexa Fluor Plus 594	FAB1060AFP594-100UG
Alexa Fluor Plus 647	FAB1060AFP647-100UG
Alexa Fluor Plus 680	FAB1060AFP680-100UG
Allophycocyanin	FAB1060A
Biotin	FAB1060B
CoraFluor 1	FAB1060CL1
DyLight 350	FAB1060D
DyLight 405	FAB1060E
DyLight 488	FAB1060K
DyLight 550	FAB1060L
DyLight 594	FAB1060M
DyLight 650	FAB1060W
DyLight 680	FAB1060Y
DyLight 755	FAB1060Z
FITC	FAB1060F
HRP	FAB1060H
Janelia Fluor 525	FAB1060JF525
Janelia Fluor 549	FAB1060I
Janelia Fluor 646	FAB1060J
Janelia Fluor 669	FAB1060JF669
PE	FAB1060P
PerCP	FAB1060C
mFluor Violet 450 SE	FAB1060MFV450
mFluor Violet 500 SE	FAB1060MFV500
mFluor Violet 610 SE	FAB1060MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB1060-SP

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NBP2-56346

Decorin Antibody - BSA Free

100 ul

NBP2-56346

Immunohistochemistry-Paraffin: Decorin Antibody [NBP2-56346] - Staining of human cerebral cortex shows low expression as expected.

Immunohistochemistry-Paraffin: Decorin Antibody [NBP2-56346] - Staining of human ovary shows high expression.

Immunohistochemistry: Decorin Antibody [NBP2-56346] - Immunohistochemical staining of human ovary shows extracellular positivity.

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Rabbit Polyclonal

Host: Rabbit Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Validated by:

Validated by:

Available Formulations

Formulation	Catalog #	Availability	Price
BSA Free	NBP2-56346-25ul

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Antibody Validation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Has Sample Size

Promotional Item

Species Glossary

Decorin Antibodies

Decorin Antibody [Unconjugated]

Decorin Antibody [Unconjugated]

Decorin Antibody (115402) [Unconjugated]

Decorin Antibody (DCN/6289)

Decorin Antibody - BSA Free

Decorin Antibody - BSA Free

Decorin Antibody (DCN/3523)

Decorin Antibody - BSA Free

Decorin Antibody (115424)

Decorin Antibody (1072716)

Decorin Antibody (161026)

Decorin Antibody - BSA Free