DEC2/SHARP1 Antibody (OTI5B1)

Western Blot: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEC2.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Human lymph node tissue.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Carcinoma of Human lung tissue.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Adenocarcinoma of Human ovary tissue.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Human pancreas tissue.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Human prostate tissue.

Immunohistochemistry: DEC2/SHARP1 Antibody (5B1) [NBP2-46574] - Analysis of Carcinoma of Human prostate tissue.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI5B1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DEC2/SHARP1 Antibody (OTI5B1) Summary

• Immunogen: Human recombinant protein fragment corresponding to amino acids 1-297 of human BHLHE41(NP_110389) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: BHLHE41
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin 1:150
  • Western Blot 1:2000
• Application Notes: For IHC-P we recommend heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120 degrees C for 3min.
• Theoretical MW: 50.3 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DEC2/SHARP1 Antibody (OTI5B1)

• basic helix-loop-helix domain containing, class B, 3
• basic helix-loop-helix family, member e41
• BHLHB3
• BHLHB3class E basic helix-loop-helix protein 41
• BHLHE41
• Class B basic helix-loop-helix protein 3
• DEC2
• DEC2Enhancer-of-split and hairy-related protein 1
• Differentially expressed in chondrocytes protein 2
• SHARP-1
• SHARP1hDEC2

Background

Human DEC1 is a 412 amino acid, basic helix-loop-helix (bHLH) containing protein that is involved in the control of proliferation and/or differentiation of several cell types including nerve cells, fibroblasts and chondrocytes. The bHLH region of DEC1 is structurally similar to the bHLH regions of the mammalian HES family, Drosophila hairy, and Enhancer of split m7. DEC1 is a novel direct target for cAMP in a wide range of cells, and is involved in the control of gene expression in cAMP-activated cells. DEC2, also known as SHARP1, is highly expressed in skeletal muscle and brain. The gene encoding human DEC2 maps to chromosome 12p11.23-p12.1. DEC1 and DEC2 play a role in regulating the mammalian molecular clock by suppressing the transcription of specific clock genes. Both DEC1 and DEC2 are detected in the suprachiasmimc nucleus in a circadian fashion. Brief light impulses induce the expression of DEC1 in a phase-dependent manner.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: BHLHE41