DDX5 Antibody

Western Blot: DDX5 Antibody [NB100-1410] - (0.3ug/ml) staining of NIH3T3 (A), HeLa (B), A431 (C) and Jurkat (D) nuclear lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: DDX5 Antibody [NB100-1410] - Paraffin embedded Human Prostate. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Data obtained using a previous batch.

Western Blot: DDX5 Antibody [NB100-1410] - DDX5 / p68 RNA helicase in Hela lysate using this antibody at 0.5 ug/ ml.

• Reactivity: Hu, Mu, Rt, Ca
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DDX5 Antibody Summary

• Description: Novus Biologicals Goat DDX5 Antibody (NB100-1410) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-DDX5 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PMIGYPMPTGYSQ corresponding to C-Terminus according to NP_004387.1.
• Predicted Species: Mouse (100%), Rat (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDX5
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 2-5 ug/ml
  • Peptide ELISA Detection Limit 1:8000
  • Western Blot 0.1-0.3 ug/ml
• Application Notes: IF: An anonymous customer has reported successful staining on HT-1080 cells with a Rhodamine conjugated secondary antibody. Data obtained from a previous batch (different goat). WB: Approx. 70 kDa band observed in nuclear lysates of NIH3T3, A431, HeLa and Jurkat (calculated MW of 69.1 kDa band according to NP_004387.1). IHC-P: Human prostate shows nuclear staining of the secretory cells in the glands. Data obtained from a previous batch (different goat).
• Control:

  DDX5 Overexpression Lysate
  A-431 Whole Cell Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX5 Antibody

• B-cell CLL/lymphoma 7 protein family member C
• B-cell CLL/lymphoma 7C
• DDX5
• DEAD/H
• G17P1
• HELR
• HLR1
• HUMP68

Background

DDX belongs to the family of DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, which are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure, such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX5 Antibody (NB100-1410)(1)

Publication using NB100-1410

• Iggo R, Gough A, Xu W et al. Chromosome mapping of the human gene encoding the 68-kDa nuclear antigen (p68) by using the polymerase chain reaction. Proc Natl Acad Sci U S A 1989-08-01 [PMID: 2762324]

Bioinformatics

• Gene Symbol: DDX5
• Entrez:
  • 1655(Human)
  • 13207(Mouse)
• Uniprot:
  • NP_004387.(Human)