DDX5 Antibody (632813)

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MCF-7 human breast cancer cell line, K562 human chronic myelogenous leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 0.1 µg/mL of Mouse Anti-Human/Mouse DDX5 Monoclonal Antibody (Catalog # MAB6370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for DDX5 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu, Mu
• Applications: WB
• Clone: 632813
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

DDX5 Antibody (632813) Summary

• Immunogen: E. coli-derived recombinant human DDX5
  Asn448-Gln614
  Accession # P17844
• Specificity: Detects human and mouse DDX5 in Western blots.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: DDX5
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDX5 Antibody (632813)

• B-cell CLL/lymphoma 7 protein family member C
• B-cell CLL/lymphoma 7C
• DDX5
• DEAD/H
• G17P1
• HELR
• HLR1
• HUMP68

Background

DDX5, also known as p68 RNA helicase, is a 614 amino acid (aa), 68 kDa member of the DEAD box family of RNA helicases that contain the conserved Asp‑Glu‑Ala‑Asp (DEAD) motif. DDX5 influences RNA transport, transcription, ribosome assembly, spliceosome function, RNA degradation, and the initiation of translation. DDX5 shares 87% aa identity with DDX17 within the central region and the two are often expressed together. The C-terminal region used as an immunogen shares 96% and 99% aa identity with mouse and rat DDX5, respectively, but lies mainly outside the DDX17 homology region.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX5 Antibody (MAB6370)(2)

Publications using MAB6370

• Goswami B, Ahuja D, Pastr� D, Ray PS. p53 and HuR combinatorially control the biphasic dynamics of microRNA-125b in response to genotoxic stress Communications Biology 2023-01-27 [PMID: 36707647]
• Goswami B, Ahuja D, Pastr� D, Ray PS. p53 and HuR combinatorially control the biphasic dynamics of microRNA-125b in response to genotoxic stress Communications Biology 2023-01-27 [PMID: 36707647]

Review for DDX5 Antibody (MAB6370) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Masashi Okada WB Human 11/04/2023

Summary

• Application: Western Blot
• Sample Tested: glioblastoma cell lysates
• Species: Human
• Lot: CEKJ012307A

Bioinformatics

• Gene Symbol: DDX5
• Uniprot:
  • P17844(Human)