DDR2 Antibody

Immunohistochemistry-Paraffin: DDR2 Antibody [NBP2-14926] - Human breast cancer, using DDR2 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Western Blot: DDR2 Antibody [NBP2-14926] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:3000.

Western Blot: DDR2 Antibody [NBP2-14926] - U87-MG whole cell and membrane extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody diluted at 1:1000.

Western Blot: DDR2 Antibody [NBP2-14926] - Mouse tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

DDR2 antibody [C2C3], C-term detects DDR2 protein at cell membrane by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DDR2 stained by DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:250.Red: Tau, an Axon marker, stained by Tau antibody [GT287] diluted at 1:500.Blue: Fluoroshield with DAPI .

Wild-type (WT) and DDR2 (KO) HeLa cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with DDR2 antibody [C2C3], C-term (NBP2-14926) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, Bv
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

DDR2 Antibody Summary

• Description: Novus Biologicals Rabbit DDR2 Antibody (NBP2-14926) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-DDR2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the C-terminus region of human DDR2. The exact sequence is proprietary.
• Predicted Species: Bovine (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDR2
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence Assay dependent
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:1000-1:10000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for DDR2 Antibody

• CD167 antigen-like family member B
• CD167b antigen
• DDR2
• Discoidin domain receptor 2
• discoidin domain receptor family, member 2
• discoidin domain receptor tyrosine kinase 2
• discoidin domain-containing receptor 2
• EC 2.7.10
• EC 2.7.10.1
• hydroxyaryl-protein kinase
• migration-inducing gene 16 protein
• neurotrophic tyrosine kinase receptor related 3
• Neurotrophic tyrosine kinase, receptor-related 3
• NTRKR3cell migration-inducing protein 20
• Receptor protein-tyrosine kinase TKT
• TKT
• TKTMIG20a
• Trk3
• TYRO10
• Tyro-10
• Tyrosine-protein kinase TYRO10
• tyrosylprotein kinase

Background

Receptor tyrosine kinases (RTKs) play a key role in the communication of cells with their microenvironment. These molecules are involved in the regulation of cell growth, differentiation, and metabolism. In several cases the biochemical mechanism by which RTKs transduce signals across the membrane has been shown to be ligand induced receptor oligomerization and subsequent intracellular phosphorylation. This autophosphorylation leads to phosphorylation of cytosolic targets as well as association with other molecules, which are involved in pleiotropic effects of signal transduction. RTKs have a tripartite structure with extracellular, transmembrane, and cytoplasmic regions. This gene encodes a member of a novel subclass of RTKs and contains a distinct extracellular region encompassing a factor VIII-like domain. Alternative splicing in the 5' UTR results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for DDR2 Antibody (NBP2-14926)(1)

Publication using NBP2-14926

• M Hulsmans, S Clauss, L Xiao, AD Aguirre, KR King, A Hanley, WJ Hucker, EM Wülfers, G Seemann, G Courties, Y Iwamoto, Y Sun, AJ Savol, HB Sager, KJ Lavine, GA Fishbein, DE Capen, N Da Silva, L Miquerol, H Wakimoto, CE Seidman, JG Seidman, RI Sadreyev, K Naxerova, RN Mitchell, D Brown, P Libby, R Weissleder, FK Swirski, P Kohl, C Vinegoni, DJ Milan, PT Ellinor, M Nahrendorf Macrophages Facilitate Electrical Conduction in the Heart Cell, 2017-04-20;169(3):510-522.e20. 2017-04-20 [PMID: 28431249]

Bioinformatics

• Gene Symbol: DDR2
• Uniprot:
  • Q16832()