DDO Antibody - BSA Free

Immunocytochemistry/Immunofluorescence: DDO Antibody [NBP1-85884] - Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex.

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human cerebral cortex, colon, lymph node and urinary bladder using Anti-DDO antibody NBP1-85884 (A) shows similar protein distribution across tissues to independent antibody NBP2-32684 (B).

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human colon.

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human lymph node.

Immunohistochemistry-Paraffin: DDO Antibody [NBP1-85884] - Staining of human urinary bladder.

Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11Lane 2: Human cell line RT-4

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DDO Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DDO Antibody - BSA Free (NBP1-85884) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: KGDWNLSPDAENSREILSRCCALEPSLHGACNIREKVGLRPYRPGVRLQTELLARDGQRLPVVHHYGHGSGGISVHWGTALEAARLVSECVHALR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  DDO Protein (NBP1-85884PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (82%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DDO Antibody - BSA Free

• aspartic oxidase
• DASOX
• D-aspartate oxidase
• DDO-1
• DDO-2
• EC 1.4.3.1
• FLJ45203

Background

OXDD (D-aspartate oxidase) is a peroxisomal flavoprotein that selectively catalyzes the oxidative deamination of D-aspartate and its N-methylated derivative, N-methyl D-aspartate. Flavin adenine dinucleotide or 6-hydroxyflavin adenine dinucleotide can serve as the cofactor in this reaction. Two transcript variants encoding different isoforms have been found.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDO