DDIT4 Overexpression Lysate (Native)

Images

 
Western Blot: DDIT4 Overexpression Lysate (Adult Normal) [NBL1-09773] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DDIT4.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DDIT4 Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC202847 and based on accession number NM_019058. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens DNA-damage-inducible transcript 4 (DDIT4), mRNA.
Gene
DDIT4

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
25.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for DDIT4

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DDIT4 Overexpression Lysate (Native)

  • Dig2
  • DNA-damage-inducible transcript 4
  • FLJ20500
  • HIF-1 responsive RTP801 (RTP801)
  • Protein regulated in development and DNA damage response 1
  • REDD-1DNA damage-inducible transcript 4 protein
  • REDD1HIF-1 responsive protein RTP801
  • RTP801RP11-442H21.1

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-240
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IP, PLA
MAB40401
Species: Hu, Mu
Applications: WB, IHC, ICC
NB100-56749
Species: Hu, Mu
Applications: WB, IHC, IHC-P
NBP2-46234
Species: Hu
Applications: WB, IHC
NB200-157
Species: Hu
Applications: WB, IHC, IHC-P
NB100-105
Species: Hu, Mu, Rt, Po, Bv, Ft, Mk, Pm, Rb, Sh, Xp, Ye
Applications: WB, ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA, TCS, KO, LA
AF2850
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP2-22127
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
NBP1-87487
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-55005
Species: Hu
Applications: WB
AF8962
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NB100-1595
Species: Hu, Mu
Applications: WB, IHC-P, IP
NBP1-89865
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89843
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-24449
Species: Hu, Mu, Rt, Pm
Applications: WB, Flow
NBL1-09773
Species: Hu
Applications: WB

Publications for DDIT4 Lysate (NBL1-09773) (0)

There are no publications for DDIT4 Lysate (NBL1-09773).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DDIT4 Lysate (NBL1-09773) (0)

There are no reviews for DDIT4 Lysate (NBL1-09773). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DDIT4 Lysate (NBL1-09773) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DDIT4 Products

Bioinformatics Tool for DDIT4 Lysate (NBL1-09773)

Discover related pathways, diseases and genes to DDIT4 Lysate (NBL1-09773). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DDIT4 Lysate (NBL1-09773)

Discover more about diseases related to DDIT4 Lysate (NBL1-09773).
 

Pathways for DDIT4 Lysate (NBL1-09773)

View related products by pathway.

PTMs for DDIT4 Lysate (NBL1-09773)

Learn more about PTMs related to DDIT4 Lysate (NBL1-09773).
 

Research Areas for DDIT4 Lysate (NBL1-09773)

Find related products by research area.

Blogs on DDIT4

There are no specific blogs for DDIT4, but you can read our latest blog posts.

Customers Who Bought This Also Bought

DDIT4 Antibody
NBP1-22966

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DDIT4 Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DDIT4