DDAH2 Antibody

Western Blot: DDAH2 Antibody [NB100-864] - Staining of Human Lung lysate with antibody at 0.1 ug/mL (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunocytochemistry/Immunofluorescence: DDAH2 Antibody [NB100-864] - Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (2 ug/mL).

Flow Cytometry: DDAH2 Antibody [NB100-864] - Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Rt, Po, Bv, Ca
• Applications: WB, ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DDAH2 Antibody Summary

• Immunogen: Peptide with sequence SSLCLVLSTRPHS corresponding to C-Terminus according to NP_039268.1.
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DDAH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.1 - 0.3 ug/mL
• Application Notes: WB: Approx. 28 kDa band observed in human lung lysates (calculated MW of 29.6 kDa band according to NP_039268.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDAH2 Antibody

• DDAHII
• dimethylargininase-2
• dimethylarginine dimethylaminohydrolase 2G6a
• dimethylarginine dimethylaminohydrolase II
• EC 3.5.3.18
• G6a
• N(G)
• N(G)-dimethylarginine dimethylaminohydrolase 2
• NG30
• NG-dimethylarginine dimethylamino hydrolase homolog
• S-phase protein

Background

DDAH2 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Overexpression of DDAH2 has also been linked to increased VEGF mRNA expression and enhanced tube formation by cells grown in a 3-dimensional medium.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDAH2 Antibody (NB100-864)(1)

Publication using NB100-864

• Leiper JM, Santa Maria J, Chubb A et al. Identification of two human dimethylarginine dimethylaminohydrolases with distinct tissue distributions and homology with microbial arginine deiminases. Biochem J 1999-10-01 [PMID: 10493931]

Bioinformatics

• Gene Symbol: DDAH2
• Entrez:
  • 23564(Human)
  • 51793(Mouse)
• Uniprot:
  • NP_039268.(Human)