DcR3/TNFRSF6B Antibody (229812)

In the presence of a cross-linking antibody, Mouse polyHistidine Monoclonal Antibody (10 µg/mL, Catalog # MAB050), Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC) inhibits Recombinant Human Fas Ligand/TNFSF6 (Catalog # 126-FL) induced apoptosis in the Jurkat human acute T cell leukemia cell line in a dose-dependent manner (orange line). Under these conditions, inhibition of Recombinant Human Fas Ligand/TNFSF6 (20 ng/mL) activity elicited by Recombinant Human DcR3/TNFRSF6B Fc Chimera (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human DcR3/TNFRSF6B Monoclonal Antibody (Catalog # MAB2135). The ND50 is typically 0.1-0.4 µg/mL.

• Reactivity: Hu
• Applications: WB, B/N
• Clone: 229812
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DcR3/TNFRSF6B Antibody (229812) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DcR3/TNFRSF6B
  Val24-His300
  Accession # O95407
• Specificity: Detects human DcR3/TNFRSF6B in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF R2, rhTRAIL R1, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF6B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Neutralization 0.1-0.4 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DcR3/TNFRSF6B Antibody (229812)

• DcR3
• DCR3Decoy receptor for Fas ligand
• Decoy receptor 3
• M68
• M68DJ583P15.1.1
• TNFRSF6B
• TR6
• TR6DcR3
• tumor necrosis factor receptor superfamily member 6B
• tumor necrosis factor receptor superfamily, member 6b, decoy

Background

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

1. Pitti, R.M. et al. (1998) Nature 396:699.
2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DcR3/TNFRSF6B Antibody (MAB2135)(1)

Publication using MAB2135

• Colucci S, Brunetti G, Mori G, Oranger A, Centonze M, Mori C, Cantatore FP, Tamma R, Rizzi R, Liso V, Zallone A, Grano M Soluble decoy receptor 3 modulates the survival and formation of osteoclasts from multiple myeloma bone disease patients. Leukemia, 2009-07-09;23(11):2139-46. 2009-07-09 [PMID: 19587706] (Neutralization, Western Blot, Human)

Bioinformatics

• Gene Symbol: TNFRSF6B
• Uniprot:
  • O95407()