DcR3/TNFRSF6B Antibody (229812) [Biotin]

• Reactivity: Hu
• Applications: ELISA(Det)
• Clone: 229812
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Biotin
• Concentration: LYOPH

DcR3/TNFRSF6B Antibody (229812) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DcR3/TNFRSF6B
  Val24-His300
  Accession # O95407
• Specificity: Detects human DcR3/TNFRSF6B in ELISAs. In sandwich immunoassays, no cross-reactivity with recombinant human (rh) Fas, rhOPG, rhTNF RII, rhTRAIL RI, rhTRAIL R2, rhTRAIL R3, or rhTRAIL R4 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: TNFRSF6B
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Human DcR3/TNFRSF6B Antibody (Catalog # MAB21351)
  ELISA Detection: Human DcR3/TNFRSF6B Biotinylated Antibody (Catalog # BAM2135)
  Standard: Recombinant Human DcR3/TNFRSF6B Fc Chimera (Catalog # 142-DC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DcR3/TNFRSF6B Antibody (229812) [Biotin]

• DcR3
• DCR3Decoy receptor for Fas ligand
• Decoy receptor 3
• M68
• M68DJ583P15.1.1
• TNFRSF6B
• TR6
• TR6DcR3
• tumor necrosis factor receptor superfamily member 6B
• tumor necrosis factor receptor superfamily, member 6b, decoy

Background

Human decoy receptor 3 (DcR3), also called TNFRSF6B, TR6 and M68, is a member of the TNF receptor superfamily. The cDNA of DcR3 encodes a 300 amino acid (aa) polypeptide with a putative 23 aa signal peptide. Like osteoprotegerin (OPG), DcR3 lacks a transmembrane sequence and is a secreted protein. DcR3 shares sequence identity with OPG (31%), TNFRII (29%) and Fas (17%). It was found to be expressed in a variety of different tissues and at high levels in many malignant tumors. Among TNF family members, DcR3 was shown to bind with Fas ligand (FasL) and LIGHT and inhibit FasL- and LIGHT-induced apoptosis. Thus, in addition to DcR1, DcR2 and OPG, DcR3 is another TNFR family molecule which modulates ligands that induce apoptosis. Overexpression of DcR3 might be a mechanism by which certain tumors escape immune-cytotoxic attack.

1. Pitti, R.M. et al. (1998) Nature 396:699.
2. Yu, K-Y. et al. (1999) J. Biochem. Chem. 274:13733.
3. Bai, C. et al. (2000) Proc. Natl. Acad. Sci. USA. 97:1230.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TNFRSF6B
• Uniprot:
  • O95407()