Daxx Antibody (DAXX-01) - BSA Free

Immunocytochemistry/Immunofluorescence: Daxx Antibody (DAXX-01) [NB500-491] - 1B - HeLa cells were co-transfected with pCDNA3-MycDaxx and pCDNA3-ASK1HA, which led to translocation of Daxx from nucleus to cytoplasm

Immunocytochemistry/Immunofluorescence: Daxx Antibody (DAXX-01) [NB500-491] - Fig. 1. Immunofluorescence staining of Daxx in transfected HeLa human cervix carcinoma cell line. Myc Daxx (green) was stained with anti-human Daxx (DAXX-01), nuclei were stained with DAPI (blue). 1A - nuclear localization of Daxx in HeLa cells transfected with pCDNA3-MycDaxx.

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: DAXX-01
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Daxx Antibody (DAXX-01) - BSA Free Summary

• Immunogen: Recombinant C-terminal part (aa 558-740) of human Daxx.
• Specificity: The antibody DAXX-01 reacts with Daxx, a death domain containing protein mainly expressed in fetal and adult human and mouse tissue.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAXX
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:10-1:2000
  • Western Blot 1:1000
• Control:

  Daxx Overexpression Lysate
  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein A purified

Alternate Names for Daxx Antibody (DAXX-01) - BSA Free

• BING2
• CENP-C binding protein
• DAP6
• DAP6MGC126245
• Daxx
• death domain-associated protein 6
• death-associated protein 6
• death-domain associated protein
• EAP1
• ETS1-associated protein 1
• Fas death domain-associated protein
• Fas-binding protein
• hDaxx
• MGC126246

Background

Daxx is an apoptosis-modulating death domain-associated protein with functions in transcriptional regulation. Daxx functions bot in cytoplasm, where it interacts with Fas, and in nucleus (residing in PML oncogenic domains), where it is involved in SUMO-dependent regulation of transcription and subnuclear compartmentalization. Daxx senzitizes the cells to apoptosis, but on the other hand, this protein may also serve in preventing apoptosis in the early embryo. Even regarding the transcription, Daxx can serve both as a corepressor and a coactivator. During ischemic stress, Daxx translocates from the nucleus to the cytoplasm, where in regulates sodium hydrogen exchanger isoform 1 (NHE1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAXX
• Entrez:
  • 1616(Human)