DAP3 Antibody - BSA Free

Western Blot: DAP3 Antibody [NB100-56325] - analysis of 10 ug of total cell lysate from Jurkat cells with this antibody.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 0.5 mg/ml

DAP3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DAP3 Antibody - BSA Free (NB100-56325) is a polyclonal antibody validated for use in WB. Anti-DAP3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The antibody was raised against a synthetic peptide corresponding to amino acids 44-57 (TSDSDPAKHGEQHE) of mouse DAP-3 protein.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAP3
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
• Application Notes: In Jurkat, a 45 kDa band is observed.
• Control:

  Jurkat Whole Cell Lysate
  DAP3 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for DAP3 Antibody - BSA Free

• bMRP-10
• DAP-3Death-associated protein 3
• death associated protein 3
• DKFZp686G12159,28S ribosomal protein S29, mitochondrial
• FLJ12817
• MGC126058
• MGC126059
• mitochondrial 28S ribosomal protein S29
• MRP-S29Ionizing radiation resistance conferring protein
• MRPS29S29mt

Background

Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Recently, five novel apoptotic genes (named DAP genes for: Death Associated Proteins) have been identified that code for proteins, which display a diverse spectrum of biochemical activities. These include a novel type of calcium/calmodulin-regulated kinase which carries ankyrin repeats and a death domain (DAP-kinase), a nucleotide-binding protein (DAP-3), a small proline-rich cytoplasmic protein (DAP-1), and a novel homolog of the eIF4G translation initiation factor (DAP-5). DAP-3 has an ATP/GTP binding motive (P-loop) and has been isolated from human and mouse and named as hDAP-3 and mDAP-3 (Death Associated Protein-3), respectively. Sequence analysis revealed an 82% amino acid identity between human and mouse protein. In situ hybridization and northern blot analysis showed an abundant mRNA expression with a pronounced expression in highly proliferative epithelial compartments. Overexpression of DAP-3 leads to cytochrome c release and induction of cell death. DAP-3 mediated apoptosis was shown to depend on a functional P-loop. mDAP-3 is localized to the mitochondrial matrix and in contrast to cytochrome c, retained its mitochondrial localization during apoptosis induction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DAP3 Antibody (NB100-56325)(1)

Publication using NB100-56325

• Berger T, Kretzler M. Interaction of DAP3 and FADD only after cellular disruption. Nat Immunol. 2002-01-01 [PMID: 11753396] (WB)
  
  Details:
  WB in various cell lines: 1. DAP3 (IMG-295), Fig 1B and 1C.

Bioinformatics

• Gene Symbol: DAP3
• Entrez:
  • 7818(Human)
  • 65111(Mouse)
  • 295238(Rat)
• Uniprot:
  • P51398(Human)