DAP12 Antibody (SR3887) - BSA Free

Western Blot: DAP12 Antibody (SR3887) [NBP3-27816] - Western blot analysis of DAP12 expression in Human PBMC cell lysate.

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC
• Clone: SR3887
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DAP12 Antibody (SR3887) - BSA Free Summary

• Description: Novus Biologicals Rabbit DAP12 Antibody (SR3887) - BSA Free (NBP3-27816) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human DAP12 (Uniprot #: O43914)
• Specificity: Detects endogenous levels of total DAP12
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TYROBP
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:80
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Western Blot 1:500-1:2000
• Theoretical MW: 12 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.4), 150mM NaCl, 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DAP12 Antibody (SR3887) - BSA Free

• DAP12
• DAP12PLOSL
• DNAX-activation protein 12
• KARAP
• KARAPPLO-SL
• KAR-associated protein
• killer activating receptor associated protein
• Killer-activating receptor-associated protein
• TYRO protein tyrosine kinase binding protein
• TYRO protein tyrosine kinase-binding protein
• TYROBP

Background

DAP12 is a transmembrane adaptor protein that is non-covalently associated with several cell surface receptors on natural killer (NK), myeloid, and presumably neuronal cells. DAP12 contains an ITAM domain that preferentially recruits the protein tyrosine kinase Syk to initiate signal transduction. Deficiency of DAP12 results in reduced antigen presentation function by myeloid cells and defects in function of certain NK cell receptors in mice, as well as presenile dementia and bone cysts in humans.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TYROBP