DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

Western Blot: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DAP Kinase 2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DAP Kinas

Immunocytochemistry/Immunofluorescence: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY DAP Kinase 2.

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Human pancreas tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Western Blot: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Analysis of extracts (35ug) from 9 different cell lines by using g anti-DAP Kinase 2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Human Kidney tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Human liver tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

Immunohistochemistry: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Staining of paraffin-embedded Human lung tissue using anti-DAP Kinase 2 mouse monoclonal antibody.

CyTOF-ready: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - Analysis of Jurkat cells, using anti-DAP Kinase 2 antibody, (Red), compared to a nonspecific negative control antibody (Blue).

CyTOF-ready: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-DAP Kinase 2 antibody, and then analyzed by flow cytometry.

Immunoprecipitation: DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free [NBP2-71753] - (Negative control: IP without adding anti-DAPK2 antibody.). For each experiment, 500ul of DDK tagged DAPK2 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-DAPK2 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

• Reactivity: Hu, Mu, Rt, Ca, Pm
• Applications: WB, Flow, ICC/IF, IHC, IP, CyTOF-ready
• Clone: OTI1C5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free (NBP2-02477) is a monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Full length human recombinant protein of human DAPK2 (NP_055141) produced in HEK293T cell.
• Isotype: IgG3
• Clonality: Monoclonal
• Host: Mouse
• Gene: DAPK2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:100
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Immunohistochemistry 1:50
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation 2ug/500ul
  • Western Blot 1:1000-2000
• Theoretical MW: 42.7 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from PBS (pH 7.3) with 8% Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for DAP Kinase 2 Antibody (OTI1C5) - Azide and BSA Free

• DAP Kinase 2
• DAPK2
• DAP-kinase-related protein 1 beta isoform
• DAP-kinase-related protein 1
• death-associated protein kinase 2
• DRP1
• DRP-1
• DRP-1MGC119312
• EC 2.7.11
• EC 2.7.11.1

Background

Protein kinase termed death-associated protein kinase 2 (DAPK2) dependant on calcium/calmodulin (Ca2+/CaM) contains an N-terminal protein kinase domain followed by a conserved CaM-binding domain with significant homologies to those of DAP kinase, a protein kinase involved in apoptosis. Overexpression of DAPK2 significantly induced the morphological changes characteristic of apoptosis. Results indicate that DAPK2 is an additional member of DAP kinase family involved in apoptotic signaling (1). The region of homology spans the catalytic domain and the CaM-regulatory region, whereas the remaining C-terminal part of the protein differs completely from DAP kinase and displays no homology to any known protein. The catalytic domain is also homologous to the recently identified ZIP kinase and to a lesser extent to the catalytic domains of DRAK1 and -2 (2). DAPK2 has a novel regulatory mechanism that controls its pro-apoptotic functions. It comprises a single autophosphorylation event mapped to Ser308 within the CaM regulatory domain. A negative charge at this site reduces both the binding to CaM and the formation of DRP-1 homodimers (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAPK2