Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line and human liver tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 37 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Simple Western lane view shows lysates of human liver tissue, loaded at 0.2 mg/mL. A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 38 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Cytosolic Sulfotransferase 1A1/SULT1A1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5546) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IP
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
  Glu2-Leu295
  Accession # P50225
• Specificity: Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SULT1E1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: SULT1A1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 25 ug/mL
  • Simple Western 10 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody [Unconjugated]

• Aryl sulfotransferase 1
• Cytosolic Sulfotransferase 1A1
• EC 2.8.2
• EC 2.8.2.1
• HAST1/HAST2
• Phenol sulfotransferase 1
• Phenol-sulfating phenol sulfotransferase 1
• P-PST 1
• P-PST
• PST
• ST1A1
• ST1A3
• STP1
• STP1MGC5163
• STPMGC131921
• sulfotransferase 1A1
• sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• SULT1A1
• Thermostable phenol sulfotransferase
• thermostable phenol sulfotransferase1
• ts-PST
• TSPST1

Background

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm (1, 2). SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity (2). The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters (3). Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers (2). The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility (4).

1. Falany, C. N. (1997) FASEB J. 11:206.
2. Gamage, N. U. et al. (2006) Toxicol. Sci. 90:5.
3. Wilborn, T.W. et al. (1993) Mol. Pharm. 43:70.
4. Gamage, N. U. et al. (2003) J. Biol. Chem. 278:7655.
5. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
6. MacRae, I.J. et al. (2000) Biochemistry 39: 1613.
7. Wu, Z.L. et al. (2002) Faseb J. 16:539.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (AF5546)(1)

Publication using AF5546

• Mebis L, Eerdekens A, Guiza F, Princen L, Derde S, Vanwijngaerden YM, Vanhorebeek I, Darras VM, Van den Berghe G, Langouche L Contribution of nutritional deficit to the pathogenesis of the nonthyroidal illness syndrome in critical illness: a rabbit model study. Endocrinology, 2011-12-13;153(2):973-84. 2011-12-13 [PMID: 22166982] (Western Blot, Rabbit)

Bioinformatics

• Gene Symbol: SULT1A1
• Uniprot:
  • P50225()