Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

Images

 
Western blot shows lysates of human liver tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Cytosolic Sulfotransferase 1E1/SULT1E1 Monoclonal Antibody (Catalog # MAB5545) followed by HRP-conjugated ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clone
726211
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211) Summary

Immunogen
E. coli-derived recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1
Asn2-Ile294
Accession # P49888
Specificity
Detects human Cytosolic Sulfotransferase 1E1/SULT1E1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross‑reactivity with recombinant human SULT1A1, 1B1, 2A1, 2B1, or 4A1 is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
SULT1E1
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
MAB5545 in the following application:

Publications
Read Publication using
MAB5545 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (726211)

  • Cytosolic Sulfotransferase 1E1
  • EC 2.8.2
  • EST-1
  • ESTMGC34459
  • estrone sulfotransferase
  • ST1E1
  • STE
  • STEestrogen sulfotransferase
  • Sulfotransferase 1E1
  • sulfotransferase family 1E, estrogen-preferring, member 1
  • Sulfotransferase, estrogen-preferringEC 2.8.2.4
  • SULT1E1

Background

Cytosolic Sulfotransferases catalyze the sulfation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1E1 is widely known as an estrogen sulfotransferase and may control estrogen levels by converting free estradiol to its inactive sulfate conjugate (1). Known substrates of this enzyme also include dehydroepiandrosterone, pregnenolone, ethinylestradiol, equalenin, diethylstilbesterol, 4‑nitrophenol, 1-napthol and resveratrol (2, 3). SULT1E1 activity is found in reproductive organs (4), intestine and liver (3, 5).
  1. Gong, H. et al. (2008) Cancer Res. 68:7386.
  2. Falany, C.N. et al. (1995) J. Steroid Biochem. Mol. Biol. 52:529.
  3. Miksits, M. et al. 2005, Xenobiotica 35:1101.
  4. Takase, Y. et al. (2007) The Prostate 67:405.
  5. Schrag, M.L. et al. (2004) Drug Metabolism and Disposition 32:1299.
  6. Robbins, P.W. (1962) Methods in Enzymology, Vol. V, Academic Press, Inc., New York, 964.
  7. MacRae, I.J., I.H. Segel, and A.J. Fisher. (2000) Biochemistry. 39: 1613.
  8. Wu, Z.L., et al. (2002) Faseb J. 16:539.
  9.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-90095
Species: Hu
Applications: IHC, IHC-P
NBP2-03227
Species: Hu
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB300-560
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
NBP1-89522
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-1596
Species: Hu, Mu, Pm, Rb, Rt
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP1-90095
Species: Hu
Applications: IHC, IHC-P
NBP2-76871
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
5829-ST
Species: Hu
Applications: EnzAct
AF6174
Species: Hu
Applications: IHC, IP, WB
AF5415
Species: Hu
Applications: ICC, IHC, Simple Western, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
NB100-1780
Species: Hu, Mu
Applications: Flow, PEP-ELISA, WB
H00347527-P01
Species: Hu
Applications: ELISA, AP, PA, WB
MAB25031
Species: Hu
Applications: IHC, IP, WB

Publications for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


Filter By Application
Western Blot
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human and Mouse.

Reviews using MAB5545:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot Cytosolic Sulfotransferase 1E1/SULT1E1 MAB5545
Enlarge
4
reviewed by:
Verified Customer
WB Human and Mouse 08/16/2021
View

Summary

ApplicationWestern Blot
Sample Testedbrain and spinal cord,Liver,Mouse brain
SpeciesHuman and Mouse
LotCGNT012107A

Comments

Comments***Novus Innovators Program - new species or application used on a primary antibody.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Cytosolic Sulfotransferase 1E1/SULT1E1 Products

Bioinformatics Tool for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)

Discover related pathways, diseases and genes to Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)

Discover more about diseases related to Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545).
 

Pathways for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)

View related products by pathway.

PTMs for Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545)

Learn more about PTMs related to Cytosolic Sulfotransferase 1E1/SULT1E1 Antibody (MAB5545).

Blogs on Cytosolic Sulfotransferase 1E1/SULT1E1

There are no specific blogs for Cytosolic Sulfotransferase 1E1/SULT1E1, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
08/16/2021
Application: WB
Species: Human and Mouse

Bioinformatics

Gene Symbol SULT1E1
Uniprot