Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (638708)

Western blot shows lysates of human liver tissue. PVDF Membrane was probed with 0.5 µg/mL of Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cytosolic Sulfotransferase 1A1/SULT1A1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Cytosolic Sulfotransferase 1A1/SULT1A1 was detected in immersion fixed paraffin-embedded sections of human brain using Human Cytosolic Sulfotransferase 1A1/SULT1A1 Monoclonal Antibody (Catalog # MAB5546) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons and glial cells (punctate). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

SULT1A1 is highly expressed in primary human monocyte-derived macrophages (MDMs). a The cellular sulfonation pathway. The first step of the cellular sulfonation pathway involves import through a sulfate transporter of a sulfate ion that is then used as a substrate by either 3′-phosphoadenosine-5′phosphosulfate (PAPS) synthetase enzymes PAPSS1 or PAPSS2. These proteins catalyze two enzymatic steps to generate PAPS, the high-energy universal sulfonate-donor from sulfate and two molecules of ATP. PAPS can be transported across the Golgi membrane and used by the Golgi sulfotransferases to generate sulfonated proteins, glycoproteins, glycoproteins, glycolipids, and proteoglycans. Alternatively, PAPS can be used by cytosolic sulfotransferases (SULTS) to sulfonate small molecules such as hormones, neurotransmitters, and xenobiotics. b Human CD4+ T cells and CD14+ monocytes were isolated from donor PBMCs by magnetic bead isolation. Resting CD4+ T cells were lysed directly after separation, and the remaining CD4+ T cells were activated using CD3/CD28 beads for three days. Monocytes were cultured for 7 days in the presence of 20 ng/ml M-CSF, were lysed, subjected to gel electrophoresis, and immunoblotting was performed to detect SULT1A1 or the loading control Ku86 protein Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/26906565), licensed under a CC-BY license. Not internally tested by R&D Systems.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clone: 638708
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (638708) Summary

• Immunogen: E. coli-derived recombinant human Cytosolic Sulfotransferase 1A1/SULT1A1
  Glu2-Leu295
  Accession # P50225
• Specificity: Detects human Cytosolic Sulfotransferase 1A1/SULT1A1 in direct ELISAs and Western blots. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) SULT1A3 and rhSULT1C4 and approximately 10% cross-reactivity with rhSULT1B1 is observed. No cross-reactivity with rhSULT1E1, 2A1, 2B1, or 4A1 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SULT1A1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation 25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Cytosolic Sulfotransferase 1A1/SULT1A1 Antibody (638708)

• Aryl sulfotransferase 1
• Cytosolic Sulfotransferase 1A1
• EC 2.8.2
• EC 2.8.2.1
• HAST1/HAST2
• Phenol sulfotransferase 1
• Phenol-sulfating phenol sulfotransferase 1
• P-PST 1
• P-PST
• PST
• ST1A1
• ST1A3
• STP1
• STP1MGC5163
• STPMGC131921
• sulfotransferase 1A1
• sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1
• SULT1A1
• Thermostable phenol sulfotransferase
• thermostable phenol sulfotransferase1
• ts-PST
• TSPST1

Background

Cytosolic Sulfotransferase 1A1 (SULT1A1; also phenol sulfotransferase 1 and thermostable phenol sulfotransferase) is a 35 kDa enzyme belonging to the sulfotransferase 1 family of proteins.  Human SULT1A1 is 295 amino acids (aa) in length.  Human SULT1A1 shares 78% and 73% aa identity with rat and mouse SULT1A1, respectively.  SULT1A1 exists as a homodimer and is expressed in liver, lung, adrenal gland, brain, platelets, and skin.  Functionally, it catalyzes the sulfate conjugation of catecholamines, phenolic drugs and neurotransmitter.  It also mediates the metabolic activation of carcinogenic N-hydroxyarylamines to DNA binding products and could participate as a modulating factor of cancer risk.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: SULT1A1
• Uniprot:
  • P50225()