Cytokeratin 8 Antibody (SR1933) - BSA Free

Immunohistochemistry: Cytokeratin 8 Antibody (SR1933) [NBP3-21664] - Immunohistochemical analysis of paraffin-embedded human liver, using NBP3-21664.

Western Blot: Cytokeratin 8 Antibody (SR1933) [NBP3-21664] - Western blot analysis using NBP3-21664 in HaCaT cell lysate.

• Reactivity: Hu, Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: SR1933
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Cytokeratin 8 Antibody (SR1933) - BSA Free Summary

• Description: Novus Biologicals Rabbit Cytokeratin 8 Antibody (SR1933) - BSA Free (NBP3-21664) is a recombinant monoclonal antibody validated for use in IHC, WB, Flow, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthesized peptide derived from human Cytokeratin 8 (Uniprot #: P05787)
• Specificity: Detects endogenous levels of total Cytokeratin 8
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT8
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:10000-1:20000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, 50% glycerol.
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for Cytokeratin 8 Antibody (SR1933) - BSA Free

• CARD2
• CK8
• CK-8
• CYK8
• Cytokeratin 8
• Cytokeratin-8
• EndoA
• K2C8
• K8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• KRT8
• Type-II keratin Kb8

Background

Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8