Cytokeratin 8/18 Antibody (KRT8.18/4997R)

Formalin-fixed, paraffin-embedded human prostate carcinoma stained with Cytokeratin 8/18 antibody (KRT8.18/4997R).18/4997R). Inset: PBS instead of primary antibody; secondary only negative control.

• Reactivity: Hu
• Applications: IHC
• Clone: KRT8.18/4997R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 8/18 Antibody (KRT8.18/4997R) Summary

• Description: Positive Controls: MCF-7 or A431 cells. Human skin, colon, lung or breast carcinoma.
  
  Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Non-hazardous. No MSDS required.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant full-length human CK8 and CK8 proteins
• Localization: Cytoplasm
• Specificity: This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stainedImmunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: 10mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (KRT8.18/4997R)

• CARD2
• CK8
• CK-8
• CYK8cytokeratin 8
• Cytokeratin-8
• K2C8
• K8cytokeratin-8
• keratin 8
• keratin, type II cytoskeletal 8
• Keratin-8
• KO
• Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8