![Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Formalin-fixed, paraffin-embedded human Overian cancer stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).](http://images.novusbio.com/fullsize2/Cytokeratin-8-18-Antibody-(K8.8-+-DC10)-Immunohistochemistry-Paraffin-NBP2-34344-img0002.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Formalin-fixed, paraffin-embedded human Overian cancer stained with Cytokeratin 8/18 Monoclonal Antibody (K8.8 + DC10).")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, Flow-IC, ICC/IF, IHC, IHC-Fr, IHC-P, IP |
| Clone | K8.8 + DC10 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10) |
| Localization | Cytoplasmic |
| Specificity | Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT8 |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Application Notes | Human Chromosome Location: 17q21.2 (CK8); 12q13.13 (CK18) Mol. Weight of Antigen: 52.5kDa (CK8); 45kDa (CK18) Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A purified |
![Choline Kinase beta [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "Choline Kinase beta [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
| Alexa Fluor 350 | NBP2-34655AF350 | |
| Alexa Fluor 405 | NBP2-34655AF405 | |
| Alexa Fluor 488 | NBP2-34655AF488 | |
| Alexa Fluor 532 | NBP2-34655AF532 | |
| Alexa Fluor 594 | NBP2-34655AF594 | |
| Alexa Fluor 647 | NBP2-34655AF647 | |
| Alexa Fluor 700 | NBP2-34655AF700 | |
| Alexa Fluor 750 | NBP2-34655AF750 | |
| Allophycocyanin | NBP2-34655APC | |
| Biotin | NBP2-34655B | |
| DyLight 350 | NBP2-34655UV | |
| DyLight 405 | NBP2-34655V | |
| DyLight 488 | NBP2-34655G | |
| DyLight 550 | NBP2-34655R | |
| DyLight 594 | NBP2-34655DL594 | |
| DyLight 650 | NBP2-34655C | |
| DyLight 680 | NBP2-34655FR | |
| DyLight 755 | NBP2-34655IR | |
| FITC | NBP2-34655F | |
| HRP | NBP2-34655H | |
| Janelia Fluor 549 | NBP2-34655JF549 | |
| Janelia Fluor 646 | NBP2-34655JF646 | |
| PE | NBP2-34655PE | |
| PerCP | NBP2-34655PCP |
Diseases for Cytokeratin 8/18 Antibody (NBP2-34344)Discover more about diseases related to Cytokeratin 8/18 Antibody (NBP2-34344).
| Pathways for Cytokeratin 8/18 Antibody (NBP2-34344)View related products by pathway.
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![Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [DyLight 550] [NBP2-34655R] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the DyLight 550 form of this antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-18-Antibody-(K8.8-+-DC10)-Flow-Cytometry-NBP2-34344-img0005.jpg "Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [DyLight 550] [NBP2-34655R] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the DyLight 550 form of this antibody.")
![Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Formalin-fixed paraffin-embedded human colon carcinoma stained with cytokeratin 8/18 Monoclonal Antibody (K8.8 +DC10)](http://images.novusbio.com/fullsize2/Cytokeratin-8-18-Antibody-(K8.8-+-DC10)-Immunohistochemistry-Paraffin-NBP2-34344-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Formalin-fixed paraffin-embedded human colon carcinoma stained with cytokeratin 8/18 Monoclonal Antibody (K8.8 +DC10)")
![Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [Alexa Fluor® 488] [NBP2-34655AF488] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-18-Antibody-(K8.8-+-DC10)-Flow-Cytometry-NBP2-34344-img0003.jpg "Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) [Alexa Fluor® 488] [NBP2-34655AF488] - An intracellular stain was performed on HeLa cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.")
![Flow (Intracellular): Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - An intracellular stain was performed on A549 cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the Azide and BSA Free form of this antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-18-Antibody-(K8.8-+-DC10)-Flow-(Intracellular)-NBP2-34344-img0004.jpg "Flow (Intracellular): Cytokeratin 8/18 Antibody (K8.8 + DC10) [NBP2-34344] - Flow Cytometry: Cytokeratin 8/18 Antibody (K8.8 + DC10) - Azide and BSA Free [NBP2-34655] - An intracellular stain was performed on A549 cells with Cytokeratin 8/18 Antibody (K8.8 + DC10) NBP2-34655R (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Dylight 550. Image using the Azide and BSA Free form of this antibody.")
![Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (DC10).](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(DC10)-Western-Blot-NBP2-29461-img0008.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Staining of human skin sweat gland. Note cytoplasmic staining of tumor cells.](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(DC10)-Immunohistochemistry-Paraffin-NBP2-29461-img0005.jpg)
![Western Blot: Cytokeratin 18 Antibody (DC10) [NBP2-29461] - Analysis using the Azide and BSA Free version of NBP2-29461. Detection of Keratin 18 in 1) HeLa and 2) A431 lysate using Keratin 18 antibody at 1 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(DC10)-Western-Blot-NBP2-29461-img0006.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Western-Blot-NBP2-47940-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47940-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47940-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Immunocytochemistry-Immunofluorescence-NBP2-34270-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).](http://images.novusbio.com/fullsize2/Cytokeratin 14 Antibody (LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0011.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Flow-Cytometry-NBP2-34270-img0009.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Western-Blot-NBP2-22194-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg)
![Western Blot: Choline kinase alpha Antibody [NBP1-85630] - Analysis in human cell lines SK-MEL-30 and U-251MG using anti-CHKA antibody. Corresponding CHKA RNA-seq data are presented for the same cell lines. Loading control: anti-HDAC1.](http://images.novusbio.com/fullsize2/Choline-kinase-alpha-Antibody-Western-Blot-NBP1-85630-img0008.jpg)
![Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human gastrointestinal shows moderate cytoplasmic positivity in glandular cells.](http://images.novusbio.com/fullsize2/Choline-kinase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85630-img0014.jpg)
![Immunohistochemistry-Paraffin: Choline kinase alpha Antibody [NBP1-85630] - Staining of human cerebral cortex shows moderate cytoplasmic positivity in neurons.](http://images.novusbio.com/fullsize2/Choline-kinase-alpha-Antibody-Immunohistochemistry-Paraffin-NBP1-85630-img0010.jpg)
![Western Blot: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of Cytokeratin 20 on different cell lysates using anti-Cytokeratin 20 antibody at 1/1,000 dilution. Positive control: Lane 1: Lovo Lane 2: HCT116 Lane 3: CRC](http://images.novusbio.com/fullsize2/Cytokeratin 20 Antibody (SA35-03)-Western Blot-NBP2-67309-img0005.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Staining Cytokeratin 20 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-(SA35-03)-Immunocytochemistry-Immunofluorescence-NBP2-67309-img0002.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody (SA35-03) [NBP2-67309] - Analysis of paraffin-embedded rat small intestine tissue using anti-Cytokeratin 20 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Cytokeratin-20-Antibody-(SA35-03)-Immunohistochemistry-Paraffin-NBP2-67309-img0004.jpg)
![Western Blot: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis using KRT10 mAb against HEK293 (1) and KRT10 (AA: 345-454)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Western-Blot-NBP2-61736-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of esophageal cancer tissues using KRT10 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Immunohistochemistry-NBP2-61736-img0004.jpg)
![Flow Cytometry: Cytokeratin 10 Antibody (3C2F5) [NBP2-61736] - Analysis of Hela cells using KRT10 mouse mAb (green) and negative control (red).](http://images.novusbio.com/fullsize2/Cytokeratin-10-Antibody-(3C2F5)-Flow-Cytometry-NBP2-61736-img0002.jpg)
![Western Blot: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of Cytokeratin 13 on human lung lysates using anti-Cytokeratin 13 antibody at 1/1,000 dilution.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-(SN71-09)-Western-Blot-NBP2-67689-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Staining Cytokeratin 13 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-(SN71-09)-Immunocytochemistry-Immunofluorescence-NBP2-67689-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody (SN71-09) [NBP2-67689] - Analysis of paraffin-embedded human tonsil tissue using anti-Cytokeratin 13 antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize2/Cytokeratin-13-Antibody-(SN71-09)-Immunohistochemistry-Paraffin-NBP2-67689-img0005.jpg)
![Dual RNAscope ISH-IHC: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Formalin-fixed paraffin-embedded tissue sections of human metastatic tonsil were probed for Pan Cytokeratin mRNA (ACD RNAScope probe, catalog # 310221; Fast Red chromogen, ACD catalog # 322500). Adjacent tissue section was processes for immunohistochemistry using mouse monoclonal (Novus catalog # NBP2-29429) at 0.3ug/mL for 1 hour at room temperature followed by incubation with the anti-mouse IgG VisUCyte HRP Polymer Antibody (Catalog # VC001) and DAB chromogen (yellow-brown). Tissue was counterstained with hematoxylin (blue).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE-1-AE-3)-Dual-RNAscope-ISH-IHC-NBP2-29429-img0015.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - MCF-7 cells stained with Biotin conjugated version of pan Cytokeratin antibody and SAv-A488. A) SAv-A488 only at a dilution of 1:500, B) pan CK at a dilution of 1:200, C) pan CK at a dilution of 1:400, D) pan CK at a dilution of 1:800. Image from verified customer review.](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE1-+-AE3)-Immunocytochemistry-Immunofluorescence-NBP2-29429-img0007.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin, pan Antibody (AE-1/AE-3) [NBP2-29429] - Analysis using Azide and BSA Free version of NBP2-29429. Human Colon Carcinoma stained with pan Cytokeratin Monoclonal Antibody cocktail (AE-1/AE3).](http://images.novusbio.com/fullsize2/Cytokeratin--pan-Antibody-(AE1-+-AE3)-Immunohistochemistry-Paraffin-NBP2-29429-img0008.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-MUC1 [SM3] at 5 ug/ml overnight at 4C and detected with an anti-mouse IgG Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/MUC-1-Antibody-(SM3)-Immunocytochemistry-Immunofluorescence-NB120-22711-img0004.jpg)
![Immunohistochemistry-Paraffin: MUC-1 Antibody (SM3) [NB120-22711] - IHC analysis of formalin fixed paraffin embedded tissue section of human breast cancer xenograft using MUC-1 antibody clone SM3 at 1:10 dilution. The xenograft section depicted a very specific and intense signal in the periphery of the cancer cells. The necrotic cells also developed a strong immune-positivity while the tumor stroma as well as the nuclei of cells were negative for immunostaining.](http://images.novusbio.com/fullsize2/MUC-1-Antibody-(SM3)-Immunohistochemistry-Paraffin-NB120-22711-img0003.jpg)
![Immunocytochemistry/Immunofluorescence: MUC-1 Antibody (SM3) [NB120-22711] - MUC-1 antibody was tested in MCF7 cells at 10 ug/ml using a Dylight 488 conjugated secondary antibody (Green). Actin (Red) and DNA (Blue) were counterstained using Phalloidin 568 and DAPI.](http://images.novusbio.com/fullsize2/MUC-1-Antibody-(SM3)-Immunocytochemistry-Immunofluorescence-NB120-22711-img0001.jpg)
![Western Blot: p53 Antibody (PAb 240) [NB200-103] - Analysis of p53 in MCF7 and HeLa lystates. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Western-Blot-NB200-103-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: p53 Antibody (PAb 240) [NB200-103] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton X-100. The cells were incubated with anti-p53 (PAb 240) [NB200-103] at a 1:200 dilution overnight at 4C and detected with an anti-mouse DyLight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunocytochemistry-Immunofluorescence-NB200-103-img0004.jpg)
![Immunohistochemistry-Paraffin: p53 Antibody (PAb 240) [NB200-103] - p53 was detected in immersion fixed paraffin-embedded sections of human prostate cancer using anti-human mouse monoclonal antibody (Catalog # NB200-103) at 1:200 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-mouse HRP polymer detection reagent (Catalog # VC001) with DAB chromogen (brown) and counterstained with hematoxylin (blue). Images may not be copied, printed or otherwise disseminated without express written permission of Novus Biologicals a bio-techne brand.](http://images.novusbio.com/fullsize2/p53-Antibody-(PAb-240)-Immunohistochemistry-Paraffin-NB200-103-img0005.jpg)
![Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]](http://images.novusbio.com/images2/Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP1-97019-img0004.jpg "Flow Cytometry: Mouse IgG Isotype Control [NBP1-97019]")