Cytokeratin 8/18 Antibody (K8.8 + DC10) [DyLight 405]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P
Clone
K8.8 + DC10
Clonality
Monoclonal
Host
Mouse
Conjugate
DyLight 405

Order Details

Cytokeratin 8/18 Antibody (K8.8 + DC10) [DyLight 405] Summary

Immunogen
Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
Localization
Cytoplasmic
Specificity
Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.Immunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Isotype
IgG
Clonality
Monoclonal
Host
Mouse
Gene
KRT8
Purity
Protein A purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Cytokeratin 8/18 Antibody (K8.8 + DC10) [DyLight 405]

  • CARD2
  • CK8
  • CK-8
  • CYK8cytokeratin 8
  • Cytokeratin-8
  • K2C8
  • K8cytokeratin-8
  • keratin 8
  • keratin, type II cytoskeletal 8
  • keratin-8
  • KO
  • Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Cytokeratin 8/18 Antibody (NBP2-34655V) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-34655AF350
Alexa Fluor 405 NBP2-34655AF405
Alexa Fluor 488 NBP2-34655AF488
Alexa Fluor 532 NBP2-34655AF532
Alexa Fluor 594 NBP2-34655AF594
Alexa Fluor 647 NBP2-34655AF647
Alexa Fluor 700 NBP2-34655AF700
Alexa Fluor 750 NBP2-34655AF750
Allophycocyanin NBP2-34655APC
Allophycocyanin/Cy7 NBP2-34655APCCY7
Biotin NBP2-34655B
DyLight 350 NBP2-34655UV
DyLight 405 NBP2-34655V
DyLight 488 NBP2-34655G
DyLight 550 NBP2-34655R
DyLight 594 NBP2-34655DL594
DyLight 650 NBP2-34655C
DyLight 680 NBP2-34655FR
DyLight 755 NBP2-34655IR
HRP NBP2-34655H
Janelia Fluor 549 NBP2-34655JF549
Janelia Fluor 646 NBP2-34655JF646
PE NBP2-34655PE
PE/Atto594 NBP2-34655PEATT594
PE/Cy5.5 NBP2-34655PECY55
PE/Cy7 NBP2-34655PECY7
PerCP NBP2-34655PCP

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Bioinformatics

Gene Symbol KRT8