Cytokeratin 8/18 Antibody (K8.8 + DC10) [PerCP]

• Reactivity: Hu
• Applications: WB, Flow, ICC/IF, IHC, CyTOF-ready
• Clone: K8.8 + DC10
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: PerCP

Cytokeratin 8/18 Antibody (K8.8 + DC10) [PerCP] Summary

• Description: This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.
• Immunogen: Keratin preparation from a human carcinoma (K8.8); PMC-42 human breast carcinoma cells (DC10)
• Localization: Cytoplasmic
• Specificity: Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This monoclonal antibody cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained. Immunohistochemical staining with this monoclonal antibody is indistinguishable from that obtained with monoclonal antibody 5D3.
• Isotype: IgG1 Kappa/IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT8
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow (Intracellular)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 8/18 Antibody (K8.8 + DC10) [PerCP]

• CARD2
• CK8
• CK-8
• CYK8cytokeratin 8
• Cytokeratin-8
• K2C8
• K8cytokeratin-8
• keratin 8
• keratin, type II cytoskeletal 8
• keratin-8
• KO
• Type-II keratin Kb8

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT8