Cytokeratin 5 Antibody (RM226)

Western Blot: Cytokeratin 5 Antibody (RM226) [NBP2-61524] - Western Blot of A431 cell lysates using NBP2-61524, showed a band of Cytokeratin 5 expressed in A431 cells.

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (RM226) [NBP2-61524] - Immunohistochemical staining of formalin fixed and paraffin embedded human Lung Squamous Cell Carcinoma tissue section using NBP2-61524 at a1:200 dilution.

Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (RM226) [NBP2-61524] - Immunohistochemical staining of formalin fixed and paraffin embedded human tonsil tissue sections.

Product Discontinued

Cytokeratin 5 Antibody (RM226) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: A peptide corresponding to the C-terminus of Human Cytokeratin 5
• Specificity: This antibody reacts to Human Cytokeratin 5. This antibody may also react to bovine, mouse, or rat Cytokeratin, as predicted by immunogen homology.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT5
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 -1:400
  • Immunohistochemistry-Paraffin 1:100 -1:400
  • Western Blot 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 50% Glycerol/PBS with 1% BSA
• Preservative: 0.09% Sodium Azide
• Purity: Protein A purified

Alternate Names for Cytokeratin 5 Antibody (RM226)

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Background

Keratins are a family of structurally related proteins that form the intermediate filament cytoskeleton in epithelial cells. The 58-kD keratin CK-5 is highly similar to other type II keratins and less similar to type I keratins and other intermediate filament proteins. The 58-kD keratin is regulated by retinoids in several tissues and is one of four keratins abundantly expressed in epidermal keratinocytes, where it may be important in maintaining structural integrity of the integument (1). Keratin 5 (CK-5) mRNA and protein are shown to be expressed in normal mammary epithelial cells in culture and are absent from tumor-derived cell lines. This makes CK-5 an important marker in the tumorigenic process, distinguishing normal from tumor cells, and decreased CK-5 expression correlates with tumorigenic progression (2). Dowling-Degos disease (DDD) is an autosomal dominant genodermatosis characterized by progressive and disfiguring reticulate hyperpigmentation of the flexures. Loss of function of CK-5 suggests a crucial role for keratins in the organization of cell adhesion, melanosome uptake, organelle transport, and nuclear anchorage (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT5