Cytokeratin 5/6 Antibody (KRT5.6/4866) - Azide and BSA Free

Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) - Azide and BSA Free [NBP3-08683] - Formalin-fixed, paraffin-embedded human squamous cell carcinoma stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

Immunohistochemistry-Paraffin: Cytokeratin 5/6 Antibody (KRT5.6/4866) - Azide and BSA Free [NBP3-08683] - Formalin-fixed, paraffin-embedded human prostate stained with Cytokeratin 5/6 Mouse Monoclonal Antibody (KRT5.6/4866).

• Reactivity: Hu
• Applications: IHC
• Clone: KRT5.6/4866
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Cytokeratin 5/6 Antibody (KRT5.6/4866) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP3-07646).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.
• Immunogen: Recombinant full-length human Cytokeratin 5/6 protein
• Localization: Cytoplasmic
• Marker: Basal, Myoepithelial & Mesothelial Cell Marker
• Specificity: It recognizes polypeptides of 58kDa and 56kDa, identified as cytokeratin 5 and 6 respectively. It shows no reaction with keratin 1, 8, or 19. It stains the basal cells of squamous and glandular epithelia, part of stratum spinosum of squamous epithelium, myoepithelium, and mesothelium. It does not stain adenous epithelium. Therefore, this antibody can be used to distinguish squamous carcinoma from adenocarcinoma, and to differentiate epitheliod mesothelioma from non-epitheliod mesothelioma and adenocarcinoma. It is also a useful antibody in the identification of mesothelioma and lung squamous cell carcinoma. It does not react with pulmonary adenocarcinoma.
• Isotype: IgG1 Kappa/IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT5
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry Formalin-fixed: 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 5/6 Antibody (KRT5.6/4866) - Azide and BSA Free

• 58 kDa cytokeratin
• CK5
• CK-5
• cytokeratin-5
• DDD
• EBS2
• epidermolysis bullosa simplex 2 Dowling-Meara/Kobner/Weber-Cockayne types
• K5
• keratin 5 (epidermolysis bullosa simplex, Dowling-Meara/Kobner/Weber-Cockaynetypes)
• keratin 5
• keratin, type II cytoskeletal 5
• Keratin-5
• KRT5A
• Type-II keratin Kb5

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT5