Cytokeratin 20 Antibody

Images

 
Western Blot: Cytokeratin 20 Antibody [NBP1-85600] - Analysis in control (vector only transfected HEK293T lysate) and KRT20 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining in human small intestine and liver tissues using anti-KRT20 antibody. Corresponding KRT20 RNA-seq data are ...read more
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining of human small intestine shows strong cytoplasmic and membranous positivity in glandular cells.
Immunohistochemistry-Paraffin: Cytokeratin 20 Antibody [NBP1-85600] - Staining of human liver shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Cytokeratin 20 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQ
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT20
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:20 - 1:50
  • Immunohistochemistry-Paraffin 1:20 - 1:50
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Control Peptide
Cytokeratin 20 Protein (NBP1-85600PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (83%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 20 Antibody

  • CD20
  • CK20
  • CK-20
  • cytokeratin-20
  • K20cytokeratin 20
  • keratin 20
  • keratin, type I cytoskeletal 20
  • keratin-20
  • KRT21
  • MGC35423
  • Protein IT

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT20