Cytokeratin 20 Antibody (10F6B12A9) - Chimeric - BSA Free

Western Blot: Cytokeratin 20 Antibody (10F6B12A9) - Chimeric - BSA Free [NBP3-43567] - Caco-2 (A) cells lysate and human colon (B) tissue lysate (35ug protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with NBP3-43567 at 1ug/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Immunocytochemistry /Immunofluorescence: Cytokeratin 20 Antibody (10F6B12A9) - Chimeric - BSA Free [NBP3-43567] - Immunofluorescence analysis of paraformaldehyde-fixed Caco-2 cells, permeabilized with 0.15% Triton, on Shi-fix coverslips stained with NBP3-43567 (1:100 dilution) for 1 hour followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing cytoplasmic and some nuclear staining. The nuclear stain is DAPI (blue). Panels show, from left to right, top to bottom, NBP3-43567, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clone: 10F6B12A9
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

Cytokeratin 20 Antibody (10F6B12A9) - Chimeric - BSA Free Summary

• Description: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format for improved compatibility with existing reagents assays and techniques.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: The original antibody was generated by immunizing female ICR mice with human CK20 peptide (amino acids 411-424) conjugated to KLH in equal volume of Freund's incomplete adjuvant. (Uniprot# P35900)
• Specificity: This antibody is specific for amino acids 411-424 of human cytokeratin 20. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. There are 19 keratin polypeptides are found in human epithelium. Cytokeratin 20 plays a significant role in maintaining keratin filament organization in intestinal epithelia. When phosphorylated, it plays a role in the secretion of mucin in the small intestine.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KRT20
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Cytokeratin 20 Antibody (10F6B12A9) - Chimeric - BSA Free

• CD20
• CK20
• CK-20
• cytokeratin-20
• K20cytokeratin 20
• keratin 20
• keratin, type I cytoskeletal 20
• keratin-20
• KRT21
• MGC35423
• Protein IT

Background

Intermediate-sized filament (IF) protein designated cytokeratin (CK) 1 is a major cellular protein of mature enterocytes and goblet cells commonly found in mucosal epithelium of the mammalian gastrointestinal tract (1). Results strongly suggest that transcriptional regulation of keratin genes in the intestinal epithelium occurs at the level of both immature and terminally differentiated epithelial cells, and is tightly regulated during both fetal development and crypt-to-villus differentiation of the intestinal epithelium (2). CK20 has recently been reported to be useful to distinguish between primary and metastatic lung adenocarcinoma. CK20 expression was significantly more prevalent in adenocarcinoma that originated in the GI tract than that of pulmonary or breast origin (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT20