![Western Blot: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/836)](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(KRT18-836)-Western-Blot-NBP2-44950-img0004.jpg "Western Blot: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Western Blot Analysis of human HCT116 cell lysate using Cytokeratin 18 Antibody (KRT18/836)")
| Reactivity | Hu, Ca, Fe, Rt(-)Species Glossary |
| Applications | WB, Flow, ICC/IF, IHC, IHC-P, PA |
| Clone | KRT18/836 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 0.2 mg/ml |
| Immunogen | Recombinant human KRT18 protein |
| Localization | Cytoplasmic |
| Marker | Epithelial Marker |
| Specificity | This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It is reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18. |
| Isotype | IgG1 |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | KRT18 |
| Purity | Protein A or G purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Hu-chromosome location: 12q13.13 Immunohistochemistry-Paraffin 2 - 4 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application. |
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| Theoretical MW | 45 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Control |
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| Storage | Store at 4C. |
| Buffer | 10 mM PBS with 0.05% BSA |
| Preservative | 0.05% Sodium Azide |
| Concentration | 0.2 mg/ml |
| Purity | Protein A or G purified |
![IL-6 [Unconjugated]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "IL-6 [Unconjugated]")
Secondary Antibodies |
Isotype Controls |
Diseases for Cytokeratin 18 Antibody (NBP2-44950)Discover more about diseases related to Cytokeratin 18 Antibody (NBP2-44950).
| Pathways for Cytokeratin 18 Antibody (NBP2-44950)View related products by pathway.
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Cytokeratin 18 - A Intermediate Filament Cyotskeletal Component Keratins, also called cytokeratins, are a family of filamentous structural proteins that form the intermediate filaments within epithelial cells. Keratins are differentially expressed depending on both the epithelial cell origin and degree of differen... Read full blog post. |
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| Gene Symbol | KRT18 |
![Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (KRT18/836) Green (CF488) and Red Dot is used to label the nuclei.](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(KRT18-836)-Immunocytochemistry-Immunofluorescence-NBP2-44950-img0002.jpg "Immunocytochemistry/Immunofluorescence: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Confocal Immunofluorescence image of HeLa cells using Cytokeratin 18 Antibody (KRT18/836) Green (CF488) and Red Dot is used to label the nuclei.")
![Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/836).](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(KRT18-836)-Immunohistochemistry-Paraffin-NBP2-44950-img0001.jpg "Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Human Prostate Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (KRT18/836).")
![Protein Array: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Analysis of Protein Array containing >19,000 full-length human proteins using Cytokeratin 18 Antibody (KRT18/836) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.](http://images.novusbio.com/fullsize2/Cytokeratin-18-Antibody-(KRT18-836)-Protein-Array-NBP2-44950-img0003.jpg "Protein Array: Cytokeratin 18 Antibody (KRT18/836) [NBP2-44950] - Analysis of Protein Array containing >19,000 full-length human proteins using Cytokeratin 18 Antibody (KRT18/836) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProt(TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProt(TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5.")
![Knockout Validated: Cytokeratin 8 Antibody [NBP2-16094] - Wild-type (WT) and Cytokeratin 8 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Cytokeratin 8 antibody. HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.](http://images.novusbio.com/fullsize2/Cytokeratin-8-Antibody-Western-Blot-NBP2-16094-img0006.jpg)
![Western Blot: Cytokeratin 8 Antibody [NBP2-16094] - Sample (30 ug of whole cell lysate) A: JC 10% SDS PAGE gel, diluted at 1:10000.](http://images.novusbio.com/fullsize2/Cytokeratin 8 Antibody-Western Blot-NBP2-16094-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 8 Antibody [NBP2-16094] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Cytokeratin 8 stained by Cytokeratin 8 antibody diluted at 1:500. Blue: Hoechst 33342 staining.](http://images.novusbio.com/fullsize2/Cytokeratin-8-Antibody-Immunocytochemistry-Immunofluorescence-NBP2-16094-img0007.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: 721_B. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0005.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - WB Suggested Anti-HOXB2 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate](http://images.novusbio.com/fullsize2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0001.jpg)
![Western Blot: HOXB2 Antibody [NBP2-83055] - Host: Rabbit. Target Name: HOXB2. Sample Type: HepG2. Antibody Dilution: 1.0ug/ml](http://images.novusbio.com/fullsize2/HOXB2-Antibody-Western-Blot-NBP2-83055-img0002.jpg)
![Simple Western: Cytokeratin 19 Antibody [NB100-687] - Lane view shows a specific band for Cytokeratin 19 in 0.5 mg/ml of HepG2 lysate. This experiment was performed under reducing A246 conditions using the 12-230 kDa separation system.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Simple-Western-NB100-687-img0008.jpg)
![Western Blot: Cytokeratin 19 Antibody [NB100-687] - Western blot detection of YAP and TAZ in proteins extracted from isolated hepatocytes and cholangiocytes and total liver tissue. Antibodies against CK19 and HNF4a were used to confirm purity of the populations and b-actin was used as a loading control. Representative results from a single experiment with n = 3 independent cell isolations and n = 2 total liver tissues. Image collected and cropped by CiteAb from the following publication(http://embomolmed.embopress.org/lookup/doi/10.15252/emmm.201506089), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Western-Blot-NB100-687-img0010.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 19 Antibody [NB100-687] - TUDCA induces intermediate states of mesenchymal and epithelial cells in primary cultures of rat HSC during hepatic differentiation. Under control conditions HSC remained negative for K19 (Cytokeratin 19) expression, (d) but this epithelial marker protein was induced in HSC cultures after treatment with 2 uM TUDCA for 14 days (red). (f) K19 was found to be co-expressed with desmin (green), which indicated the origin of epithelial progenitor cells from HSC. (I) Cells with different states of maturation were still found after 21 days of TUDCA treatment. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep13320), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Cytokeratin-19-Antibody-Immunocytochemistry-Immunofluorescence-NB100-687-img0013.jpg)
![Western Blot: Vimentin Antibody [NB300-223] - Analysis of tissue and cell lysates. Antibody at 1:5000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. NB300-223 binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, dilution 1:5000 in green, revealing multiple bands around 280 kDa that correspond to full length MAP2A/2B isotypes, and ~70 kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Western-Blot-NB300-223-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Vimentin Antibody [NB300-223] - Comparative analysis of epithelial and mesenchymal markers expressions. Fluorescence immunocytochemistry staining for E-cadherin (green), N-cadherin (red) and vimentin (cyan) was performed on esophageal cancer cells under static and microfluidic flow conditions at different time points. Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/srep38221), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin Antibody-Immunocytochemistry-Immunofluorescence-NB300-223-img0008.jpg)
![Immunohistochemistry: Vimentin Antibody [NB300-223] - Increased numbers of differentiating NSPCs in SVZ of G93A-SOD1 brain. Confocal microscopy images of dorsal and ventral regions of SVZ in G93A-SOD1 and Wt-SOD1 brain at postnatal week 18, stained for nestin (green), GFAP (red), and vimentin (blue). Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (http://www.molecularbrain.com/content/8/1/5), licensed under a CC-BY licence.](http://images.novusbio.com/fullsize2/Vimentin-Antibody-Immunohistochemistry-NB300-223-img0007.jpg)
![Western Blot: Albumin Antibody [NB600-41532] - Analysis using the HRP conjugate of NB600-41532. Detection of Albumin in whole mouse liver extracts. Image courtesy of anonymous customer product review.](http://images.novusbio.com/fullsize2/Albumin-Antibody-Western-Blot-NB600-41532-img0002.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Immunofluorescence Analysis of A549 cells labeling CK14 with Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot (Red).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Immunocytochemistry-Immunofluorescence-NBP2-34270-img0010.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Antibody (LL002).](http://images.novusbio.com/fullsize2/Cytokeratin 14 Antibody (LL002)-Immunohistochemistry-Paraffin-NBP2-34270-img0011.jpg)
![Flow Cytometry: Cytokeratin 14 Antibody (LL002) [NBP2-34270] - Flow Cytometric Analysis of trypsinized MeOH-fixed HeLa cells using Cytokeratin 14 Antibody (LL002) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).](http://images.novusbio.com/fullsize2/Cytokeratin-14-Antibody-(LL002)-Flow-Cytometry-NBP2-34270-img0009.jpg)
![Western Blot: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Western Blot Analysis of human HeLa cell lysate using Cytokeratin 7 Antibody (OV-TL12/30).](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Western-Blot-NBP2-47940-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Immunofluorescent staining of methanol-fixed HeLa cells. Cytokeratin 7 Antibody (OV-TL12/30) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Red Dot.](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-47940-img0006.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 7 Antibody (OV-TL12/30) - Azide and BSA Free [NBP2-47940] - Human Lung SCC stained with Cytokeratin 7 Monoclonal Antibody (OV-TL12/30)](http://images.novusbio.com/fullsize2/Cytokeratin-7-Antibody-(OV-TL12-30)---Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47940-img0009.jpg)
![Western Blot: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Image of Caspase-3 Antibody (31A1067) - (Pro and Active). Whole cell protein from Jurkat cells treated with and without 2 uM staurosporine as indicated was separated on a 4-15% gel by SDS-PAGE, transferred to 0.2 um PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 5 ug/ml anti-Caspase 3 in 1% milk, and detected with an anti-mouse HRP secondary antibody using a Femto sensitivity chemiluminescence reagent. Note the detection of both pro-caspase 3 at 35 kDa and the cleaved active caspase 3 at 15-17 kDa.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Western-Blot-NB100-56708-img0031.jpg)
![Immunohistochemistry-Paraffin: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Tissue section of human spleen using 1:200 dilution of Caspase-3 antibody (clone 31A1067). The staining was developed with HRP labeled anti-mouse IgG secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This Caspase 3 antibody generated primarily a specific cytoplasmic staining in a subset of spleenocytes with some nuclear signal in a few cells.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Immunohistochemistry-Paraffin-NB100-56708-img0042.jpg)
![Simple Western: Caspase-3 Antibody (31A1067) - (Pro and Active) [NB100-56708] - Simple Western lane view shows a specific band for Caspase-3 Antibody (31A1067) - (Pro and Active) in 0.1 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230kDa separation system.](http://images.novusbio.com/fullsize2/Caspase-3-Antibody-(31A1067)---(Pro-and-Active)-Simple-Western-NB100-56708-img0033.jpg)
![Western Blot: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis using Cytokeratin 5 mouse mAb against A431 (1), MCF-7 (2), HeLa (3), HepG2 (4), 3T3-L1 (5), and COS-7 (6) cell lysate.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Western-Blot-NBP2-22194-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunocytochemistry-Immunofluorescence-NBP2-22194-img0003.jpg)
![Immunohistochemistry-Paraffin: Cytokeratin 5 Antibody (2C2) [NBP2-22194] - Analysis of stomach cancer tissues using Cytokeratin 5 mouse mAb with DAB staining.](http://images.novusbio.com/fullsize2/Cytokeratin-5-Antibody-(2C2)-Immunohistochemistry-Paraffin-NBP2-22194-img0005.jpg)
![SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.](http://images.novusbio.com/images2/Mouse-IgG1-Isotype-Control-SDS-Page-NBP1-97005-img0002.jpg "SDS-Page: Mouse IgG1 Isotype Control [NBP1-97005] - Lane 1:, Non-reduced. M: Opal Pre-stained Ladder. Lane 2:, Reduced. Load: 1.0 ug per lane. Predicted/Observed: 120 kDa Non-reduced, 55 and 25 Reduced.")