Cytokeratin 18 Antibody (C-04 (same as Ks18.04))

Immunohistochemistry-Paraffin: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [NBP2-44951] - Human Thyroid Carcinoma stained with Cytokeratin 18 Monoclonal Antibody (C-04).

Flow Cytometry: Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) [NBP2-44951] - Flow Cytometric Analysis of PFA-fixed HeLa cells using Cytokeratin 18 Antibody (C-04 (same as Ks18.04))followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red)

• Reactivity: Hu, Mu, Rt, Po, Ca, Eq, Fe, Gt, Ha, Ma, Pm, Pm, Sh, Ch(-), Xp(-)
• Applications: Flow, IHC, IHC-P, ICC/IF
• Clone: C-04 (same as Ks18.04)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Cytokeratin 18 Antibody (C-04 (same as Ks18.04)) Summary

• Immunogen: Cytoskeletal preparation extracted from the human vulvar squamous carcinoma cell line A431.
• Localization: Cytoplasmic
• Specificity: This MAb reacts with a wide variety of simple epithelia. It does not react with stratified squamous epithelia. It reacts with epithelial tumors of the gastrointestinal tract, lung, breast, pancreas, ovary, and thyroid. Cytokeratin 18, which belongs to the type A (acidic) subfamily of low molecular weight keratins, exists in combination with cytokeratin 8. It was reported that tissues from gastrointestinal tract are positive for both cytokeratin 8 and 18 but do not contain cytokeratin 14. Tissues from gastrointestinal tract, respiratory tract and urogenital tract, as well as endocrine and exocrine tissues and mesothelial cells are positive for cytokeratin 18.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: KRT18
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 0.5 - 1 ug/million cells in 0.1 ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5 - 1.0 ug/ml
  • Immunofluorescence 1 - 2 ug/ml
• Application Notes: Hu-chromosome location: 12q13.13
  Immunohistochemistry-Paraffin 0.5 - 1 ug/ml for 30 minutes at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10 - 20 min followed by cooling at RT for 20 minutes. The 7mL size is a pre-diluted size and no additional dilutions are required before using this item for the intended application.
• Theoretical MW: 45 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Cytokeratin 18 Knockout HeLa Cell Lysate

Reactivity Notes

Human, Mouse, Rat, Hamster, ferret, Cow, Pig, Cat, Dog, Sheep, Goat, Horse, Marmoset Monkey, Does not react with chicken, Xenopus. Others not known.

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Cytokeratin 18 Antibody (C-04 (same as Ks18.04))

• Cell proliferation-inducing gene 46 protein
• cell proliferation-inducing protein 46
• CK-18
• CYK18
• Cytokeratin 18
• cytokeratin-18
• K18
• Keratin 18
• keratin, type I cytoskeletal 18
• keratin-18
• KRT18

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KRT18