Cytokeratin 15 Antibody

Images

 
Orthogonal Strategies: Western Blot: Cytokeratin 15 Antibody [NBP1-85603] - Analysis in human cell lines A-431 and HEK293 using Anti-KRT15 antibody. Corresponding KRT15 RNA-seq data are presented for the same ...read more
Immunocytochemistry/ Immunofluorescence: Cytokeratin 15 Antibody [NBP1-85603] - Staining of human cell line HaCaT shows localization to intermediate filaments.
Orthogonal Strategies: Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85603] - Staining in human esophagus and stomach tissues using anti-KRT15 antibody. Corresponding KRT15 RNA-seq data are ...read more
Western Blot: Cytokeratin 15 Antibody [NBP1-85603] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-143
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85603] - Staining of human esophagus shows high expression.
Immunohistochemistry-Paraffin: Cytokeratin 15 Antibody [NBP1-85603] - Staining of human stomach shows low expression as expected.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

Cytokeratin 15 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: RLEQEIATYRSLLEGQDAKMAGIGIREASSGGGGSSSNFHINVEESVDGQVVSSHKREI
Specificity
Specificity of human Cytokeratin 15 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
KRT15
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.4 ug/ml
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
Cytokeratin 15 Protein (NBP1-85603PEP)

Reactivity Notes

Expected species cross reactivity based on sequence homology: Mouse (80%)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 15 Antibody

  • CK15
  • CK-15
  • cytokeratin 15
  • Cytokeratin-15
  • K15keratin-15, basic
  • K1CO
  • keratin 15
  • keratin, type I cytoskeletal 15
  • Keratin-15
  • keratin-15, beta
  • KRTB
  • type I cytoskeletal 15

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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ICC/IF Video Protocol

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Bioinformatics

Gene Symbol KRT15