Cytokeratin 13 Antibody

Images

 
Western Blot: Cytokeratin 13 Antibody [NBP1-06047] - (0.1ug/ml) staining of Human Lung lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Immunohistochemistry-Paraffin: Cytokeratin 13 Antibody [NBP1-06047] (2.5ug/ml) staining of paraffin embedded Human Tonsil. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Cytokeratin 13 Antibody Summary

Immunogen
Peptide with sequence C-TSNASGRRTSDVRRP corresponding to C-Terminus according to NP_705694.2.
Epitope
C-TSNASGRRTSDVRRP
Specificity
This antibody is expected to recognize the reported isoform a (NP_002265.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KRT13
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 - 0.3 ug/ml
  • Immunohistochemistry 2 - 4 ug/ml
  • Immunohistochemistry-Paraffin 2 - 4 ug/ml
  • Peptide ELISA Detection limit 1:128000
Application Notes
WB: Approx. 55 kDa band observed in human lung, skeletal muscle and skin lysates is reported in scientific literature (PMID: 12686638). IHC-P: Human tonsil shows strong layered, filamentous staining.
Positive Control
Cytokeratin 13 Lysate (NBL1-12380)
Publications
Read Publications using NBP1-06047.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Cytokeratin 13 Antibody

  • CK13
  • CK-13
  • cytokeratin 13
  • Cytokeratin-13
  • K13cytokeratin-13
  • keratin 13
  • keratin, type I cytoskeletal 13
  • keratin-13
  • MGC161462
  • MGC3781

Background

The protein encoded by this gene is a member of the keratin gene family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Most of the type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This type I cytokeratin is paired with keratin 4 and expressed in the suprabasal layers of non-cornified stratified epithelia. Mutations in this gene and keratin 4 have been associated with the autosomal dominant disorder White Sponge Nevus. The type I cytokeratins are clustered in a region of chromosome 17q21.2. Alternative splicing of this gene results in multiple transcript variants; however, not all variants have been described. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Cytokeratin 13 Antibody (NBP1-06047)(2)

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol KRT13
Entrez
Uniprot